SignalSilence? NDRG1 siRNA I

• 产品编号：CST-6245S      品牌：CST       原厂货号：6245S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® NDRG1 siRNA I可以帮助研究者通过RNA干扰特异性地抑制NDRG1的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® β-Catenin siRNA II进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。N-myc下游调控gene1（NDRG1），也被称为Cap43，Drg1，RTP/rit42和Proxy-1，是NDRG家族的一员，该家族包含的4个成员（NDRG1-4）在生长，分化和细胞存活过程中发挥作用（1-5）。广泛表达的NDRG1并对多种压力信号发生应激反应，包括DNA损伤（4），缺氧（5），和镍及钙浓度升高（2）。NDRG1在N-myc缺陷小鼠中表达升高，能够被N-和c-myc负调控（1-6）。在DNA损伤过程中，NDRG1通过p53信号激活，并且是p53介导的凋亡过程中所必须的（4,7）。研究显示，NDRG1通过促进分化，抑制生长，调控血管生成和肿瘤转移等进程中发挥功能（3,4,6,8,9）。NDRG1基因发生无义突变后会引发遗传性运动和感觉神经病病变-Lom(HMSNL),该结论由证明NDRG1在维持髓鞘和轴突存活的研究支持（10,11）。NDRG1在肥大细胞成熟过程中表达上调，它的缺失会导致衰减的过敏反应（12）。NDRG1和NDRG2都是SGK1的底物，尽管SGK1介导的磷酸化的生理性作用尚未可知（13）。NDRG1可以被SGK1在Thr328, Ser330, Thr346, Thr356,以及Thr366磷酸化。NDRG1被SGK1磷酸化后可以被GSK-3磷酸化。

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

原厂资料：

Description

SignalSilence® NDRG1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit NDRG1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM NDRG1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use

Background

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

注意事项：

Storage: NDRG1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.