SignalSilence? Toll-like Receptor 3 siRNA I

• 产品编号：CST-6236S      品牌：CST       原厂货号：6236S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST生产的SignalSilence® Toll-like Receptor 3 siRNA I可以通过特异性的扰特Toll-like Receptor 3的表达，此方法是通过传递双链RNA到细胞中从而选择性的沉默目的基因。所有的SignalSilence® siRNA 产品经过严格的公司内部测试并通过Western验证可有效的降低蛋白的表达。寡聚核苷酸的合成是在三苯甲游基的控制下保持适当的结合效率。寡聚链随后通过亲和固相提纯。退火的双链RNA通过质谱进一步的分析以确定抽提的成分。每一个批次都通过质谱跟前一个批次比较以确定批次间的连贯性。CST推荐转染条件为：转染100 nM Toll-like Receptor 3 siRNA I，48-72小时后裂解细胞。至于转染的步骤需根据转染试剂的方法手册进行。如有问题请联系我们。

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.

原厂资料：

Description

SignalSilence® Toll-like Receptor 3 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Toll-like Receptor 3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Toll-like Receptor 3 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.

注意事项：

Storage: Toll-like Receptor 3 siRNA I is supplied in RNAse-free
water. Aliquot and store at -20ºC.