SignalSilence? β-Arrestin 1 siRNA I

• 产品编号：CST-6218S      品牌：CST       原厂货号：6218S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® β-Arrestin 1 siRNA I 可以帮助研究者通过RNA干扰特异性地抑制β-Arrestin 1的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^®siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® β-Arrestin 1 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Arrestin蛋白的作用是G蛋白偶联受体（GPCR）信号传导的负调节因子，同源配体的结合刺激了GPCR的磷酸化，随后Arrestin蛋白结合到磷酸化的GPCR上，导致受体的内化和GPCR信号传导的脱敏(1)。目前已在四种不同的哺乳动物体内发现了Arrestin蛋白，Arrestin1（也被称为S-arrestin）、arrestin 4 (或成为 X-arrestin) 分别存在于视网膜的视杆细胞和视锥细胞中。Arrestin 2 (也被称为β-arrestin 1) and arrestin 3 (或成为 β-arrestin 2) 广泛表达于各种组织，它们结合到多数GPCR上(2)。β-arrestin 蛋白作为适配器和支架蛋白发挥作用，此外该蛋白也参与其他反应，如通过ERK活化途径将c-Src蛋白家族聚集到GPCRs上 (3,4)。β-arrestins也参与一些受体酪氨酸激酶信号传导途径(5-8)。其他证据暗示β-arrestin蛋白能转移到细胞核内，并通过结合到转录辅助因子上而调节转录(9,10)。

1. Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10.
2. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7.
3. Luttrell, L.M. et al. (1999) Science 283, 655-61.
4. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83.
5. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65.
6. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23.
7. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8.
8. Waters, C.M. et al. (2005) Cell Signal 17, 263-77.
9. Kang, J. et al. (2005) Cell 123, 833-47.
10. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8.

原厂资料：

Description

SignalSilence® β-Arrestin 1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit β-arrestin 1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM β-Arrestin 1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (or β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).

1. Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10.
2. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7.
3. Luttrell, L.M. et al. (1999) Science 283, 655-61.
4. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83.
5. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65.
6. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23.
7. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8.
8. Waters, C.M. et al. (2005) Cell Signal 17, 263-77.
9. Kang, J. et al. (2005) Cell 123, 833-47.
10. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8.

注意事项：

Storage: β-Arrestin 1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.