SignalSilence? ILK1 siRNA I

• 产品编号：CST-6202S      品牌：CST       原厂货号：6202S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

ILK1 siRNA I会抑制人、小鼠和大鼠的ILK1 表达。来自Cell Signaling Technology (CST)公司的SignalSilence® ILK1 siRNA I让研究者用RNA 干扰技术特异抑制ILK1表达，这是一种通过发送双链RNA分子到细胞选择性抑制基因表达方法。所有SignalSilence®siRNA产物来自CST，经过严格的内部测试，并由western analysis已经显示减少蛋白表达。CST 推荐使用100nM ILK1siRNA I转染，48到72小时后裂解细胞。对应转染步骤，按转染试剂生产商提供的步骤进行，如在使用上有任何问题请随时联系CST。整合素连接酶(ILKs)配对整合素和生长因子到下游通路涉及细胞存活，周期控制，细胞与细胞粘附和细胞移动（1）。ILK作为一个支架，通过与PINCH（此蛋白通过Nck2连接ILK到RTKs），CH-ILKBP 和affixin相互作用，桥连细胞外基质(ECM)和生长因子受体到肌动蛋白骨架（1）。ILK磷酸化Akt的473位丝氨酸，磷酸化GSK-3 的9位丝氨酸，磷酸化肌球蛋白轻链2（MLC2）的18位丝氨酸/19位苏氨酸，还有affixin（2-5）。这些磷酸化时调控靶标活性的关键调控步骤。ILK活性被PI3激酶刺激，被肿瘤抑制因子PTEN和PP2C蛋白磷酸酶ILKAP负向调控（1,3,6）。已经暗示，保守的343位丝氨酸残基在激活环中对ILK1的激活起关键作用（2）。小分子干扰RNA (siRNA)已经在HEK-293细胞中被用于特异沉默ILK表达（7）。

1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.
7. Troussard, A. A. et al. (2003) J. Biol. Chem. 278(25) , 22374-22378.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

ILK1 siRNA I will inhibit human, mouse and rat ILK1 expression.

Description

SignalSilence® ILK1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ILK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM ILK1 siRNA I 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).

1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.
7. Troussard, A. A. et al. (2003) J. Biol. Chem. 278(25) , 22374-22378.

注意事项：

Storage: ILK1 siRNA I is supplied in RNase-free water. Aliquot
and store at –20°C.