干扰是一种通过向细胞中输入双链RNA分子，选择性沉默基因表达的方法。一种特定的小干扰RNA的引入可以通过降解RNA来阻止目的蛋白的翻译。SignalSilence ® Control siRNA （荧光标记）是一种小干扰RNA（siRNA），不能导致细胞信号的特定降解，而用来作为靶siRNA转染的阴性对照。另外，这种siRNA带荧光标记，可以使实验者通过荧光显微镜评估转染效率。建议裂解细胞前用100nM Control siRNA II 转染48-72个小时。请参考转染试剂生产商提供的转染步骤。如有问题，请联系CST公司。
原厂资料：
Description
RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence® Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.
Directions for Use
CST recommends transfection with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
注意事项：
Storage: SignalSilence Control siRNA (Fluorescein Conjugate)
is supplied in RNAse-free water. Aliquot and store at -20°C.
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