c-Jun Fusion Protein

• 产品编号：CST-6093S      品牌：CST       原厂货号：6093S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 激活剂
• 应用分类：

描述：

c-Jun融合蛋白是SAPK/JNK蛋白的有效底物，SAPK/JNK蛋白能磷酸化其Ser63、Ser73位点。它是一种重组的融合蛋白，对应于c-Jun蛋白密码子1-89区域氨基酸残基。在体外激酶分析实验中，c-Jun融合蛋白在20 µl反应体系中的浓度为2 µg/20 µl，能被有活力的 SAPK蛋白磷酸化，使用的试剂是1X Kinase Buffer (#9802) 和 200 µM ATP (#9804)。30ºC反应30分钟后，用免疫印迹法就能检测到磷酸化，使用的抗体是phospho-specific c-Jun antibodies兔多抗 (#9164 and #9261)。

c-Jun是Jun家族的一个成员，Jun家族成员包括c-Jun, JunB, 和 JunD。c-Jun是转录因子激活蛋白-1（AP-1）的一个组分。AP-1是由Fos和Jun形成的二聚体以及 ATF家族成员组成的，能够结合并激活TRE/AP-1元件的转录（1）。包括生长因子，细胞因子和压力在内的细胞外信号能够激活AP-1依赖的转录过程。C-Jun的转录活性是通过SAPK/JNK对其中的Ser63和Ser73的磷酸化来调控的（2）。小鼠敲除实验表明c-Jun对于胚胎形成是至关重要的（3），进一步的研究发现c-Jun在多种组织和发育过程中发挥作用，包括轴突再生（4），肝脏再生（5）和T细胞发育（6）。AP-1所调控的基因发挥了多种生物学功能，包括细胞增殖、分化、凋亡、转化，侵润和转移，这依赖于细胞种类和具体情况（7-9）。其他的AP-1所调控的靶基因还能够调节细胞存亡，以及低氧和血管生成（8,10）。研究提示c-Jun可以作为癌症、血管重构、急性炎症反应和风湿性关节炎的重要治疗靶点（11,12）。

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

原厂资料：

Description

c-Jun Fusion Protein serves as a useful substrate for SAPK/JNK, which will phosphorylate it at Ser63 and Ser73 (1). It is expressed as a recombinant protein fusion to amino acid residues corresponding to c-Jun codons 1-89.

Directions for Use

c-Jun Fusion Protein at a concentration of 0.5 µg/µl in a 20 µl reaction can be phosphorylated using active SAPK in an in vitro kinase assay with 1X Kinase Buffer (#9802) and 200 µM ATP (#9804). After a 30-minute assay at 30ºC, phosphorylation can be detected by Western blot with phospho-specific c-Jun antibodies (#9164 and #9261).

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

注意事项：

Storage: Supplied in 20 mM Tris-HCl (pH 7.5 at 25°C), 50
mM NaCl, 2 mM Na2EDTA, 1 mM dithiothreitol (DTT) and 50%
glycerol. Store at –20°C.