Streptavidin (Magnetic Bead Conjugate)

• 产品编号：CST-5947S      品牌：CST       原厂货号：5947S
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 亲和素、链霉亲和素、生物素 > 链霉亲和素
• 应用分类：

描述：

链霉亲和素与其自然配体生物素有极高的结合能力。这种复合体及其非传统的生物素结合位点的结构使其对生物素的结合有较高的选择性，并能展现亲和素与生物素复合体的特异性。链霉亲和素在中表达。Streptavidin (Magnetic Bead Conjugate)能用于生物素化的蛋白预沉淀（1，2）。重组的链霉亲和素与氨基酸群结合的甲酰基芳酰胺磁珠相互结合被固化。添加10μl 涡旋过的珠子至在1 x 1毫克/毫升1X Cell Lysis Buffer (10X) #9803中的200μl细胞裂解产物。更多细节请参见使用手册。为了免疫沉淀复合体的进一步洗脱和磁珠的清洗, 可用6-Tube Magnetic Separation Rack #7017分开磁珠。将包含磁珠的试管放置在磁力架上,等待1-2分钟，仔细去除上清。将试管重新取下,添加新的溶液，通过轻轻地涡旋与震荡试管重新悬浮磁珠。链霉亲和素是从细菌纯化得到的53000道尔顿四聚物的蛋白质(3)。每个子单元与生物素都有极高的结合力。由于它与生物素无交叉反应,它可被用来隔离生物素化的蛋白质(1、2)。

原厂资料：

Specificity / Sensitivity

Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

Source / Purification

Streptavidin is expressed in Escherichia coli.

Description

Streptavidin (Magnetic Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized by the covalent binding of primary amino groups with formylbenzamide-modified magnetic bead.

Directions for Use

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.

Background

Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).

注意事项：

Storage: Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20.
Store at 4ºC. Do not aliquot the antibody