Human Interleukin-5 (hIL-5)

• 产品编号：CST-5924SF      品牌：CST       原厂货号：5924SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

重组人白介素-5(hIL-5) Ile20-Ser134 (Accession #NP_000870) 由CST公司在人293T细胞中进行表达。重组hIL-5没有标签，非糖基化的蛋白分子量为13,149Da。DTT还原蛋白接近14-21kDa多肽的迁移位置。由于糖基化使其在SDS-PAGE中有异质性和较低的迁移率。非还原的胱氨酸连接的同源二聚体接近32-38kDa蛋白的迁移位置。预期的重组hIL-5氨基端IPTEI通过氨基酸测序验证。用6 μg还原的(+)和非还原的(-)重组hIL-5进行SDS-PAGE，纯度都大于98%。所有批次的产品纯度都大于98%。在丁酸诱导分化的HL-60 Clone 15细胞中通过磷酸-p44/42 MAPK (Erk1/2)的定量来评估hIL-5的活性。ED50值在0.5-1ng/ml之间。每微克hIL-5内毒素含量小于0.01ng。

有载体：将含有hIL-5的PBS溶液（pH7.2，每1 μg hIL-5中含20 μg BSA）经0.22μm滤膜过滤并冻干。

无载体：将含有hIL-5的PBS溶液（pH7.2）经0.22μm滤膜过滤并冻干。

IL-5（白细胞介素-5）是一种主要由TH2 T细胞产生的细胞因子，有活性的嗜酸性粒细胞、肥大细胞、NK细胞和iNK细胞也能产生（1-4）。人和小鼠的IL-5都是糖基化的二硫键连接的同源二聚体（1）。IL-5受体是异源二聚体，包含一个高亲和力的IL-5结合α链和公共β链（与GM-CSF和IL-3受体共享）来进行信号转导（1,2）。可溶性IL-5Rα对IL-5有高亲和力，因此能抑制IL-5活性（1）。IL-5介导的信令能激活Erk1/2，Jak2和Stat5信号通路（1,2）。在小鼠中，IL-5对细胞从活化的B细胞分化成抗体分泌型细胞有重要作用（2）。在人和小鼠中，IL-5都能诱导嗜酸性粒细胞活化、增殖和分化（2,3）。

原厂资料：

Source / Purification

Recombinant human IL-5 (hIL-5) Ile20-Ser134 (Accession #NP_000870) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-5 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,149. DTT-reduced protein migrates as a 14-21 kDa polypeptide. Heterogeneity and lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-38 kDa protein. The expected amino-terminal IPTEI of recombinant hIL-5 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-5. All lots are greater than 98% pure.

Bioactivity

The activity of hIL-5 was assessed by quantification of phospho-p44/42 MAPK (Erk1/2) in butyric acid differentiated HL-60 Clone 15 cells. The observed ED50 values are between 0.50-1 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-5.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-5. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-5 is a pleiotrophic cytokine that is predominantly produced by TH2 T cells but can also be expressed by activated eosinophils, mast cells, NK cells, and iNKT cells (1-4). Both human and mouse IL-5 are glycosylated disulfide-linked homodimers (1). The IL-5 receptor is a heterodimer that consists of a high affinity IL-5 binding α chain and the common β chain, which is shared by GM-CSF and IL-3 receptors, for signal transduction (1,2). Soluble IL-5Rα binds with high affinity to IL-5, thereby inhibiting IL-5 activity (1). IL-5-mediated signaling can activate the Erk1/2, Jak2, and Stat5 signaling pathways (1,2). In mice, IL-5 is important for the differentiation of antibody secreting cells from activated B cells (2). IL-5 induces eosinophil activation, proliferation, and differentiation in both mice and humans (2,3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.