SOS1 Antibody

• 产品编号：CST-5890S      品牌：CST       原厂货号：5890S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SOS1 Antibody兔多抗能检测内源性总SOS1蛋白水平。该多克隆抗体通过使用与人源SOS1蛋白lle1198位点周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。交换因子（SOS）是果蝇中最早发现的Ras鸟苷酸交换因子，作为Sevenless 受体的下游（1）。哺乳动物细胞中发现了2个果蝇SOS的同源物：SOS1和SOS2（2）。SOS1含有组氨酸折叠，Dbl（DH）和pleckstrin（PH）同源结构域，一个Ras交换基序（REM）和Cdc25同源物和多脯氨酸结构域（3）。SOS1和GRB2、NCK和其他受体蛋白结合，在ERK激活下游受体酪氨酸激酶受体(RTK)的过程中发挥了重要作用。研究发现了Noonan综合征病人的SOS1基因发生突变，这是一个以身材矮小、面部变形和先天性心脏病为特征的疾病（4,5）。

1. Rogge, R.D. et al. (1991) Cell 64, 39-48.
2. Nimnual, A. and Bar-Sagi, D. (2002) Sci STKE 2002, pe36.
3. Chardin, P. et al. (1993) Science 260, 1338-43.
4. Tartaglia, M. et al. (2007) Nat Genet 39, 75-9.
5. Roberts, A.E. et al. (2007) Nat Genet 39, 70-4.

原厂资料：

Specificity / Sensitivity

SOS1 Antibody detects endogenous levels of total SOS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1198 of human SOS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Son of sevenless (SOS) was first identified in Drosophila as a guanine nucleotide exchange factor (GEF) for Ras acting downstream of the Sevenless receptor (1). Two closely related homologs of Drosophila SOS are found in mammalian cells: SOS1 and SOS2 (2). SOS1 consists of histone folds, Dbl (DH) and pleckstrin (PH) homology domains, a Ras exchange motif (REM), and Cdc25 homology and polyproline domains (3). SOS1 binds to GRB2, NCK and other adaptor proteins, and plays an important role in ERK activation downstream of protein tyrosine kinase receptor (RTK). Mutations in the corresponding SOS1 gene have been found in people with Noonan syndrome, a developmental disorder characterized by short stature, facial dysmorphia and congenital heart defects (4,5).

1. Rogge, R.D. et al. (1991) Cell 64, 39-48.
2. Nimnual, A. and Bar-Sagi, D. (2002) Sci STKE 2002, pe36.
3. Chardin, P. et al. (1993) Science 260, 1338-43.
4. Tartaglia, M. et al. (2007) Nat Genet 39, 75-9.
5. Roberts, A.E. et al. (2007) Nat Genet 39, 70-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.