Bmf (G81) Antibody

• 产品编号：CST-5889S      品牌：CST       原厂货号：5889S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bmf (G81) Antibody 能够检测内源性水平Bmf总蛋白。该多克隆抗体是采用合成的人源Bmf蛋白Gly81周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。BH3-only蛋白是Bcl-2家族促凋亡蛋白的分支，均有保守的BH3结构域，但缺乏BH1，BH2，BH4（1）。这个短的BH3结构域，对于与Bcl-2家族促生存成员的互动是必不可少的，使其具有促凋亡活性。在哺乳动物中，已经鉴定了大量的BH3-only蛋白，包括Bmf、Bad、Bik、Bid、Bim、Hrk、Noxa和Puma。这些蛋白质大多通过组织特异性表达在细胞凋亡中扮演不同的角色。最初，Bmf（BCL-2 修饰因子）是在酵母双杂交筛选中用促生存蛋白Mcl-1作为诱饵鉴定的（2）。研究表明在多种细胞系中检测到Bmf的广泛表达，如B和T淋巴细胞，以及髓系或成纤维起源和所有发育阶段的小鼠胚胎中。 bmf的蛋白在胰腺，肝脏，肾脏和造血组织最为丰富（2，3）。Bmf能够与很多促生存Bcl-2蛋白（包括Mcl-1，Bcl-2，Bcl-xL和Bcl-w）发生相互作用，这种作用依赖于BH3结构域（2）。 据报道，与Bim一样，Bmf结合到细胞骨架上。Bmf通常通过与动力蛋白轻链2（DLC2）相互作用螯合到肌球蛋白V马达。某些损伤信号，如从脱离基质的粘附细胞系（失巢凋亡），触发了Bmf的释放，进而与促生存Bcl-2蛋白结合（2）。

1. Puthalakath, H. and Strasser, A. (2002) Cell Death Diff. 9, 505-512.
2. Puthalakath, H. et al. (2001) Science 293, 1829-1832.
3. Morales, A. A. et al. (2004) Leukemia 18, 41-47.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Bmf (G81) Antibody recognizes endogenous levels of total Bmf protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of human Bmf protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The BH3-only proteins are a group of pro-apoptotic proteins of the Bcl-2 family that share the conserved BH3 domain but lack BH1, BH2, and BH4 (1). This short BH3 domain is essential for interaction with pro-survival members of the Bcl-2 family and allows for their pro-apoptotic activity. A large number of BH3-only proteins have been identified in mammals, including Bmf, Bad, Bik, Bid, Bim, Hrk, Noxa, and Puma. Many of these proteins appear to display distinct roles in apoptosis through tissue-specific expression. Bmf (Bcl-2-modifiying factor) was originally identified in a yeast two-hybrid screen using the pro-survival protein Mcl-1 as bait (2). Bmf appears to be widely expressed, with bmf mRNA observed in cell lines of B- and T-lymphoid, myeloid, or fibroblastoid origin and in mouse embryos at all developmental stages. Bmf protein is seen most abundantly in pancreas, liver, kidney, and hematopoietic tissues (2, 3). Bmf interacts with several pro-survival Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-xL, and Bcl-w, and the interaction depends on the BH3 domain (2). Like Bim, Bmf has been reported to bind to cytoskeletal structures. Bmf is normally sequestered to myosin V motors through association with dynein light chain 2 (DLC2). Certain damage signals, such as the detachment of adherent cell lines from their substratum (anoikis), triggers the release of Bmf and subsequent binding to the pro-surivival Bcl-2 proteins (2).

1. Puthalakath, H. and Strasser, A. (2002) Cell Death Diff. 9, 505-512.
2. Puthalakath, H. et al. (2001) Science 293, 1829-1832.
3. Morales, A. A. et al. (2004) Leukemia 18, 41-47.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.