GRK6 (D1A4) Rabbit mAb

• 产品编号：CST-5878S      品牌：CST       原厂货号：5878S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

<、p>GRK6 (D1A4) Rabbit mAb能够检测内源水平GRK6蛋白总体水平。此单克隆抗体是通过合成人源对应的GRK6 Glu89位点周围的肽段来免疫动物而获得。G蛋白偶联受体激酶6 (GRK6)是7个GRK丝氨酸/苏氨酸激酶亚家族成员中的一个, 他们主要的作用是使激活的G蛋白偶联受体(GPCRs)脱敏(1,2)。GRKs作用是通过磷酸化激活的GPCRs丝氨酸/苏氨酸残基。磷酸化这些残基后作为β-arrestin蛋白的结合位点, 他们能够通过阻止G蛋白的再偶联而抑制GPCRs的再激活(3)。有证据表明，GRKs也能调节非GPCR信号通路(2)。 例如, GRK6能通过磷酸化LRP6而调节Wnt 信号通路(4)和胰岛素样生长因子信号通路(5)。GRK6可能在天然免疫系统中也起了作用。研究者发现GRK6的表达在造血细胞系肿瘤中非常丰富, 最近的研究表明GRK6抑制能够选择性的对骨髓瘤细胞系致死 (6)。

1. Mushegian, A. et al. (2012) PLoS One 7, e33806.
2. Gurevich, E.V. et al. (2012) Pharmacol Ther 133, 40-69.
3. Ribas, C. et al. (2007) Biochim Biophys Acta 1768, 913-22.
4. Chen, M. et al. (2009) J Biol Chem 284, 35040-8.
5. Zheng, H. et al. (2012) Proc Natl Acad Sci U S A 109, 7055-60.
6. Tiedemann, R.E. et al. (2010) Blood 115, 1594-604.

原厂资料：

Specificity / Sensitivity

GRK6 (D1A4) Rabbit mAb recognizes endogenous levels of total GRK6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu89 of human GRK6 protein.

Background

G protein-coupled Receptor Kinase 6 (GRK6) is one of 7 members of the GRK serine/threonine kinase subfamily, which are known primarily for their role in desensitizing activated G protein-coupled receptors (GPCRs) (1,2). GRKs function by phosphorylating serine/threonine residues in activated GPCRs. Upon phosphorylation these residues serve as binding sites for β-arrestin proteins, inhibiting re-activation of GPCRs by blocking their re-association with G proteins (3). There is evidence that GRKs can also modulate selected non-GPCR signaling pathways (2). For example, GRK6 has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (4), and the insulin-like growth factor signaling pathway (5). GRK6 may also play a role in immune system function. Investigators have found GRK6 expression is typically abundant in hematopoietic tumor cell lines, and a recent research study demonstrated that GRK6 suppression was selectively lethal for a number of myeloma tumor cell lines (6).

1. Mushegian, A. et al. (2012) PLoS One 7, e33806.
2. Gurevich, E.V. et al. (2012) Pharmacol Ther 133, 40-69.
3. Ribas, C. et al. (2007) Biochim Biophys Acta 1768, 913-22.
4. Chen, M. et al. (2009) J Biol Chem 284, 35040-8.
5. Zheng, H. et al. (2012) Proc Natl Acad Sci U S A 109, 7055-60.
6. Tiedemann, R.E. et al. (2010) Blood 115, 1594-604.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody