PSA/KLK3 (D2A8) Rabbit mAb

• 产品编号：CST-5877S      品牌：CST       原厂货号：5877S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PSA/KLK3 (D2A8) Rabbit mAb用于检测内源性PSA/KLK3总蛋白。该单克隆抗体由人PSA/KLK3蛋白110位苯丙氨酸附近残基合成的多肽免疫动物产生。激肽释放酶3（KLK3）, 即公认的前列腺特异性抗原，是丝氨酸蛋白酶亚家族—腺体激肽释放酶的成员之一(1)。KLK3由前列腺上皮细胞产生，并被分泌到前列腺管中。7个N-末端的氨基酸断裂后(2)，KLK3在精子凝块中被活化以液化精液。尽管PSA/KLK3存在于健康人体内，但研究者也发现在恶性程度高的前列腺癌患者的血液中可以检测到高滴度的PSA/KLK3 (3,4)。

1. Watt, K.W. et al. (1986) Proc Natl Acad Sci U S A 83, 3166-70.
2. Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
3. Thompson, I.M. et al. (2004) N Engl J Med 350, 2239-46.

原厂资料：

Specificity / Sensitivity

PSA/KLK3 (D2A8) Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe110 of human PSA/ KLK3 protein.

Background

Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4).

1. Watt, K.W. et al. (1986) Proc Natl Acad Sci U S A 83, 3166-70.
2. Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
3. Thompson, I.M. et al. (2004) N Engl J Med 350, 2239-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody