Rat Vascular Endothelial Growth Factor-164 (rVEGF₁₆₄ )

• 产品编号：CST-5874LC      品牌：CST       原厂货号：5874LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > VEGF血管内皮生长因子
• 应用分类：

描述：

Cell Signaling Technology在人293细胞中表达生产重组大鼠鼠蛋白VEGF164 (rVEGF164) Ala27-Arg190 (Accession #NP_001103803)。重组的rVEGF164蛋白不包含标签，没有糖基化的蛋白分子量据推算为19,234Da。DTT-还原的蛋白迁移时是作为一个24-31 kDa 的多肽而转移。在SDS-PAGE中的低迁移率是由于其糖基化的作用。未经还原的胱氨酸连接的同型二聚体迁移时作为46-53 kDa 的蛋白而迁移。重组rVEGF164蛋白的N-末端APTTE序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hVEGF164通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白rVEGF164 的生物活性是同过HUVEC的细胞增殖实验确定的。每个批次的ED50在1-5 ng/ml之间。内毒素含量:<0.01 ng 内毒素/1 μg rVEGF164。

有载体: 每微克hVEGF164蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hVEGF164蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

VEGF164是VEGF-A基因众多剪切方式中的一种,并且之比其人源配对物VEGF165少一个氨基酸(1, 2)。能产生VEGF164的细胞有很多，包括内皮细胞，巨噬细胞和T细胞(1, 2)。VEGF164与血管生成，血管内皮细胞的存活，生长，迁移，和血管渗透性等过程有关(1,2)。基因受到缺氧，炎症细胞因子以及原癌基因的诱导而表达(1, 2)。VEGF164结合到硫酸乙酰肝素并保留在细胞表面和细胞外基质(1-3)。VEGFR1 和 VEGFR2是VEGF164的受体酪氨酸激酶(2)。NRP-1和NRP-2作为其共受体并增强VEGFR2的信号(2-3)。VEGF164结合到VEGFR1 和VEGFR2 上导致PI3K/AKT, p38 MAPK, FAK 和 Paxillin的激活(2)。

原厂资料：

Source / Purification

Recombinant rat VEGF164 (rVEGF164) Ala27-Arg190 (Accession #NP_001103803) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant rVEGF164 contains no "tags" and the nonglycosylated protein has a calculated MW of 19,234. DTT-reduced protein migrates as a 24-31 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 46-53 kDa protein. The expected amino-terminal APTTE of recombinant rVEGF164 was verified by amino acid sequencing.

Bioactivity

The bioactivity of recombinant rVEGF164 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg rVEGF164. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

VEGF164 is one of many splice variants of the VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1, 2). VEGF164 is produced by a number of cells including endothelial cells, macrophages, and T cells (1, 2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration, and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines, and oncogenes (1, 2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK, and Paxillin (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.