CA12 (D75C6) XP? Rabbit mAb

• 产品编号：CST-5864P      品牌：Cell Signaling Technology       原厂货号：5864P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CA12 (D75C6) XP® Rabbit mAb兔单抗用于检测内源性CA12总蛋白水平。该单抗通过用对应于人源CA12蛋白Gly39位点周围残基序列的合成肽免疫动物制备而得。碳酸酐酶(CA)是一种古老的锌金属酶家族，存在于几乎所有的生物中。CA可以分成三个大类(α, β, 和γ)，它们是独立进化的，并且在序列和总体折叠方式上也有很大不同。功能性的CA在它的活性位点都有一个锌原子，这对于它的催化作用是必需的，它在CO2水化形成HCO3-和H+的化学反应中起着可逆的催化作用。哺乳类的CA有很多种的同工酶，它们都隶属α类CA(1,2)。CA12是碳酸酐酶α类别蛋白，它是一种膜蛋白，催化结构域位于胞外。CA12在多种组织中广泛表达，在肾细胞癌中高表达。它的表达在实体瘤中被低氧所诱导，因此被当作是一个肿瘤标志物（3-5）。CA12的基因缺陷跟高色汗症有关（6,7）。

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
3. Chiche, J. et al. (2009) Cancer Res 69, 358-68.
4. Barnett, D.H. et al. (2008) Cancer Res 68, 3505-15.
5. Watson, P.H. et al. (2003) Br J Cancer 88, 1065-70.
6. Feldshtein, M. et al. (2010) Am J Hum Genet 87, 713-20.
7. Muhammad, E. et al. (2011) Hum Genet 129, 397-405.

原厂资料：

Specificity / Sensitivity

CA12 (D75C6) XP® Rabbit mAb detects endogenous levels of total CA12 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of human CA12 protein.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).CA12, a member of the α class, is a plasma membrane protein with the catalytic domain in the extracellular space. CA12 is widely expressed in many tissues and is found at high levels in renal cell cancers. Its expression is induced by hypoxia in solid tumors and is considered to be a tumor marker (3-5). Genetic mutations in CA12 are associated with hyperchlorhidrosis (6,7).

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
3. Chiche, J. et al. (2009) Cancer Res 69, 358-68.
4. Barnett, D.H. et al. (2008) Cancer Res 68, 3505-15.
5. Watson, P.H. et al. (2003) Br J Cancer 88, 1065-70.
6. Feldshtein, M. et al. (2010) Am J Hum Genet 87, 713-20.
7. Muhammad, E. et al. (2011) Hum Genet 129, 397-405.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody