NFAT1 (D43B1) XP? Rabbit mAb

• 产品编号：CST-5861S      品牌：CST       原厂货号：5861S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NFAT1 (D43B1) XP® Rabbit mAb兔单抗能够检测内源NFAT1蛋白总体水平。此单克隆抗体是通过合成人源对应的NFAT1 Gly87位点周围的肽段来免疫动物而获得。NFAT (T细胞激活的核因子)家族蛋白包括 NFAT1 (NFATc2或 NFATp), NFAT2 (NFATc1 或 NFATc), NFAT3 (NFATc4)和 NFAT4 (NFATc3 或NFATx)。此家族中的所有成员都是转录因子，具有一个Rel同源的区域和与AP-1 (Jun/Fos)相呼应的调控基因转录，从而管理形成高效的免疫系统(1,2)。NFAT 蛋白主要是在免疫系统的细胞中表达，但是也在骨骼肌，角化细胞，脂肪细胞中表达并调控这些细胞的分化过程(3)。在静息细胞中, NFAT蛋白被高度磷酸化并在细胞质中定位。细胞内的钙浓度增加能激活钙/钙调素依赖的丝氨酸磷酸酶，它能够激活NFAT蛋白，导致其随后的入核(2)。终止NFAT的信号发生在降低钙的浓度和通过GSK-3或CK1对NFAT的磷酸化后(3,4)。环孢菌素A 和 FK506 是免疫抑制剂能够抑制钙依赖磷酸酶并还原胞质中的NFAT蛋白(5)。

1. Northrop, J.P. et al. (1993) J Biol Chem 268, 2917-23.
2. Hogan, P.G. et al. (2003) Genes Dev 17, 2205-32.
3. Crabtree, G.R. and Olson, E.N. (2002) Cell 109 Suppl, S67-79.
4. Okamura, H. et al. (2004) Mol Cell Biol 24, 4184-95.
5. Shaw, K.T. et al. (1995) Proc Natl Acad Sci U S A 92, 11205-9.

原厂资料：

Specificity / Sensitivity

NFAT1 (D43B1) XP® Rabbit mAb detects endogenous levels of total NFAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly87 of human NFAT1 protein.

Background

The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5).

1. Northrop, J.P. et al. (1993) J Biol Chem 268, 2917-23.
2. Hogan, P.G. et al. (2003) Genes Dev 17, 2205-32.
3. Crabtree, G.R. and Olson, E.N. (2002) Cell 109 Suppl, S67-79.
4. Okamura, H. et al. (2004) Mol Cell Biol 24, 4184-95.
5. Shaw, K.T. et al. (1995) Proc Natl Acad Sci U S A 92, 11205-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.