DBC1 (3G4) Mouse mAb

• 产品编号：CST-5857S      品牌：CST       原厂货号：5857S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DBC1 (3G4) Mouse mAb鼠单抗能够检测内源性DBC1总蛋白水平。通过重组人源DBC1蛋白氨基端去免疫动物从而制备出此单克隆抗体。Deleted in breast cancer gene 1 protein (DBC1)最初通过它定位到染色体8p21区域被鉴定，它在乳腺癌中纯合子的缺失(1)。在细胞存活中，DBC1蛋白是一个具有多种功能的分子量大的细胞核蛋白。它以非配体依赖性方式直接结合到雌激素受体 (strogen receptor α，ERα)激素结合区域，并且可能是非配体ERα 表达和存在人源乳腺癌细胞系中的一个关键因素(2)。DBC1蛋白可能通过结合到和抑制SirT1蛋白的去乙酰酶活性从而促进p53介导的凋亡，这导致p53乙酰化水平和活性的增加(3)。DBC1蛋白可能是一个重要异染色质形成的调节因子，因为它结合SUV39H1以及抑制它的组蛋白甲基转移酶活性(4)。在Tumor Necrosis Factor-α (TNF-α)介导的细胞死亡信号期间，Caspase依赖的进程可以激活DBC1的促凋亡活性(5)。在TNF-α刺激下，DBC1蛋白的这个过程发生在凋亡的初期，并且导致DBC1蛋白重新定位到细胞质。DBC1蛋白的加工的细胞质形式的过量表达导致线粒体的群集和基质浓缩，并且使的细胞对于TNF-α介导凋亡敏感。

1. Hamaguchi, M. et al. (2002) Proc Natl Acad Sci USA 99, 13647-52.
2. Trauernicht, A.M. et al. (2007) Mol Endocrinol 21, 1526-36.
3. Zhao, W. et al. (2008) Nature 451, 587-90.
4. Li, Z. et al. (2009) J Biol Chem 284, 10361-6.
5. Sundararajan, R. et al. (2005) Oncogene 24, 4908-20.

原厂资料：

Specificity / Sensitivity

DBC1 (3G4) Mouse mAb recognizes endogenous levels of total DBC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DBC1 protein.

Background

Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor α (ERα) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERα expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-α (TNF-α)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-α is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Overexpression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-α-mediated apoptosis.

1. Hamaguchi, M. et al. (2002) Proc Natl Acad Sci USA 99, 13647-52.
2. Trauernicht, A.M. et al. (2007) Mol Endocrinol 21, 1526-36.
3. Zhao, W. et al. (2008) Nature 451, 587-90.
4. Li, Z. et al. (2009) J Biol Chem 284, 10361-6.
5. Sundararajan, R. et al. (2005) Oncogene 24, 4908-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody