HO-1 (D60G11) Rabbit mAb

• 产品编号：CST-5853S      品牌：CST       原厂货号：5853S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HO-1 (D60G11) Rabbit mAb用于检测内源性HO-1总蛋白水平。该单抗通过用对应于人源HO-1蛋白Ala110位点周围残基序列的合成肽免疫动物制备而得。血红素氧合酶（HO）是血红素分解代谢过程中的限速酶，该反应会释放一氧化碳，铁离子和胆绿素（1）。该反应的产物对多种生理功能如抗氧化反应，抗炎症反应和细胞保护功能至关重要（2）。HO包括两种同工酶，包括组成型表达的HO-2和诱导型的HO-1（3）。在受到一些刺激，包括血红素，金属离子和激素等，诱导型的HO-1会表达（4）。HO-1的诱导表达跟多种疾病有关，如移植排斥，高血压，动脉硬化，Alzheimer病，内毒素休克，糖尿病，炎症和神经疾病等（1,5）。

1. Abraham, N.G. and Kappas, A. (2008) Pharmacol Rev 60, 79-127.
2. Otterbein, L.E. et al. (2003) Trends Immunol 24, 449-55.
3. Cruse, I. and Maines, M.D. (1988) J Biol Chem 263, 3348-53.
4. Maines, M.D. (1988) FASEB J 2, 2557-68.
5. Schipper, H.M. et al. (2009) Curr Alzheimer Res 6, 424-30.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

HO-1 (D60G11) Rabbit mAb recognizes endogenous levels of total HO-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of human HO-1 protein.

Background

Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).

1. Abraham, N.G. and Kappas, A. (2008) Pharmacol Rev 60, 79-127.
2. Otterbein, L.E. et al. (2003) Trends Immunol 24, 449-55.
3. Cruse, I. and Maines, M.D. (1988) J Biol Chem 263, 3348-53.
4. Maines, M.D. (1988) FASEB J 2, 2557-68.
5. Schipper, H.M. et al. (2009) Curr Alzheimer Res 6, 424-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody