NDRG3 Antibody

• 产品编号：CST-5846S      品牌：CST       原厂货号：5846S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NDRG3抗体识别内源性的NDRG3蛋白。多克隆抗体由合成的肽段免疫动物产生。其合成的肽段与人源NDRG3蛋白邻近羧基末端的氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析法纯化。NDRG（N-Myc下游调控基因）家族，由NDRG1, NDRG2, NDRG3, and NDRG4组成，是一组与结构调节相关的蛋白，在细胞增殖、分化、凋亡和应激反应、细胞迁移/转移中起作用[1-3]。NDRG1最初在N-Myc基因敲除小鼠中被鉴定为一种上调的蛋白[1]。NDRG家族蛋白，特别是NDRG1和NDRG2被报道在各种癌症组织中下调表达，提示其功能角色为肿瘤抑制因子[4,5].NDRG的家族成员的表达具有差异性；NDRG3大部分在睾丸、前列腺、卵巢和大脑中表达[2,6,7]。也有报道NDRG3在前列腺细胞系中因雄激素作用而上调，并在那些中促进细胞增殖[8]。

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
6. Okuda, T. and Kondoh, H. (1999) Biochem Biophys Res Commun 266, 208-15.
7. Zhao, W. et al. (2001) Biochim Biophys Acta 1519, 134-8.
8. Wang, W. et al. (2009) Int J Cancer 124, 521-30.

原厂资料：

Specificity / Sensitivity

NDRG3 Antibody recognizes endogenous levels of total NDRG3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The NDRG (N-Myc downstream-regulated gene) family, consisting of NDRG1, NDRG2, NDRG3, and NDRG4, are a group of structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was up-regulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues, and have been suggested to function as a tumor suppressors (4,5).Members of the NDRG family each have distinct expression patterns; NDRG3 is most highly expressed in the testis, prostate, ovary, and brain (2,6,7). NDRG3 has also been shown to be up-regulated by androgen in prostate cell lines and can promote cell growth in those cells (8).

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
6. Okuda, T. and Kondoh, H. (1999) Biochem Biophys Res Commun 266, 208-15.
7. Zhao, W. et al. (2001) Biochim Biophys Acta 1519, 134-8.
8. Wang, W. et al. (2009) Int J Cancer 124, 521-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody