GAD2 (D5G2) XP? Rabbit mAb

• 产品编号：CST-5843S      品牌：CST       原厂货号：5843S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GAD2 (D5G2)XP® Rabbit mAb 兔单抗识别内源性的总GAD2蛋白。该单克隆抗体由合成的人类GAD2蛋白羧基末端附近肽段免疫动物而制成。谷氨酸脱羧酶（GAD）负责从L-谷氨酸合成重要的神经递质γ-氨基丁酸（GABA）（1）。GAD1（GAD67）和GAD2（GAD65）在神经系统和内分泌系统表达（2），被认为分别参与突触传递（3）和胰岛素分泌（4）。GAD2的自身抗体可作为I型糖尿病的生物标记之一（5）。许多患成年期发病的僵人综合症（SPS）的患者也表达抗体GAD2（6）。

1. Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
2. Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
3. Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
4. Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
5. Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
6. Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.

原厂资料：

Specificity / Sensitivity

GAD2 (D5G2) XP® Rabbit mAb recognizes endogenous levels of total GAD2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAD2 protein.

Background

The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).

1. Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
2. Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
3. Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
4. Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
5. Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
6. Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody