EGF Receptor (E746-A750del Specific) (D6B6) XP? Rabbit mAb (Biotinylated)

• 产品编号：CST-5747S      品牌：CST       原厂货号：5747S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EGF Receptor (E746-A750缺失特异) (6B6) XP® 兔单抗(生物素标记)能够检测内源性水平的EGFR E746-A750缺失突变蛋白。该单克隆抗体是通过用合成肽免疫动物而制备的，该合成肽是人EGFR的E746-A750缺失突变序列。该Cell Signaling Technology抗体在最佳条件下与生物素结合。该生物素化抗体预期与未结合的EGF Receptor (E746-A750del Specific) (6B6) XP® 兔单抗 #2085表现出同一物种的交叉反应。表皮生长因子（EGF）受体是一种170kDa的跨膜酪氨酸激酶，属于HER/ErbB蛋白家族。调查研究表明，EGF受体（EGFR）酪氨酸激酶结构域的体细胞突变存在于非小细胞肺癌子集，即对EGFR抑制剂吉非替尼、厄洛替尼有反应的子集（1-3）。有两种类型的突变占突变的情况约90％：一个特定的点突变，在外显子21的L858R突变和外显子19中的框内缺失（4,5）。虽然出现一些罕见的变异，但最常见的19号外显子缺失是E746-A750，占该位点病变的90％。

1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

原厂资料：

Specificity / Sensitivity

EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated) detects endogenous levels of EGFR E746-A750del mutant protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb #2085.

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot antibody.