Notch2 (D76A6) XP? Rabbit mAb

• 产品编号：CST-5732S      品牌：CST       原厂货号：5732S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Notch2 (D76A6) XP^® 兔单抗识别内源性的Notch2蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Notch2蛋白的Ala2378氨基酸残基序列一致。Notch1是一个在细胞发育和命运决定过程中发挥功能的跨膜蛋白（1）。在notch1成熟过程中，notch1分子细胞外结构域会被一个成对碱性氨基酸蛋白酶样的转化酶剪切（2）。在与配体如Delta1结合或是细胞外钙离子耗竭后，notch1的羧基端被释放并随后在Gly1743 和 Val1744之间被切开（3,4）。结果导致细胞内处于活化状态的notch1片段转运至细胞核内并转录激活。Notch2是Notch家族成员之一，Notch2的突变和Alagille综合征有关(5).

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.

原厂资料：

Specificity / Sensitivity

Notch2 (D76A6) XP^® Rabbit mAb recognizes endogenous levels of total Notch2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein.

Background

Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (5).

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody