ELP3 (D5H12) Rabbit mAb

• 产品编号：CST-5728S      品牌：CST       原厂货号：5728S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ELP3 (D5H12) Rabbit mAb检测内源性ELP3总蛋白。通过人工合成人源ELP3蛋白Gly431位点周围相应的多肽片段去免疫动物从而制备出此单克隆抗体。

Elongator是一个高度保守的转录延伸因子复合物，它首先在酵母中被鉴定，并且作为高度磷酸化的RNA polymerase II (RNAPII)全酶的部分(1)。Elongator复合物是由6个亚单位ELP1-6组成，并且研究显示有乙酰化转移酶活性 (2)。Elongator乙酰化靶点包含 histone H3和α-tubulin，而histone H3是联系着该复合物的转录延伸功能，以及α-tubulin是与迁移的调节和投射神经元的成熟有关(3-6)。

ELP3是Elongator复合物的催化乙酰转移酶亚单位(2,3)。ELP3 包含一个铁硫簇，它能结合S-adenosylmethionine，这对于Elongator复合物完整性是必要的，并且研究显示在介导父系基因组受精作用之后的整个受精卵DNA去甲基化(7-9)。

1. Otero, G. et al. (1999) Mol Cell 3, 109-18.
2. Creppe, C. and Buschbeck, M. (2011) J Biomed Biotechnol 2011, 924898.
3. Wittschieben, B.O. et al. (1999) Mol Cell 4, 123-8.
4. Hawkes, N.A. et al. (2002) J Biol Chem 277, 3047-52.
5. Kim, J.H. et al. (2002) Proc Natl Acad Sci USA 99, 1241-6.
6. Creppe, C. et al. (2009) Cell 136, 551-64.
7. Paraskevopoulou, C. et al. (2006) Mol Microbiol 59, 795-806.
8. Greenwood, C. et al. (2009) J Biol Chem 284, 141-9.
9. Okada, Y. et al. (2010) Nature 463, 554-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Horse

Specificity / Sensitivity

ELP3 (D5H12) Rabbit mAb recognizes endogenous levels of total ELP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly431 of human ELP3 protein.

Background

Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6).

ELP3 is the catalytic acetyltransferase subunit of the Elongator complex (2,3). ELP3 contains an iron-sulfur cluster that can bind S-adenosylmethionine, which is necessary for the structural integrity of the Elongator and has been shown to play an important role in mediating global zygotic DNA demethylation of the paternal genome post-fertilization (7-9).

1. Otero, G. et al. (1999) Mol Cell 3, 109-18.
2. Creppe, C. and Buschbeck, M. (2011) J Biomed Biotechnol 2011, 924898.
3. Wittschieben, B.O. et al. (1999) Mol Cell 4, 123-8.
4. Hawkes, N.A. et al. (2002) J Biol Chem 277, 3047-52.
5. Kim, J.H. et al. (2002) Proc Natl Acad Sci USA 99, 1241-6.
6. Creppe, C. et al. (2009) Cell 136, 551-64.
7. Paraskevopoulou, C. et al. (2006) Mol Microbiol 59, 795-806.
8. Greenwood, C. et al. (2009) J Biol Chem 284, 141-9.
9. Okada, Y. et al. (2010) Nature 463, 554-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody