Mouse His6Interleukin-27 (mHis6IL-27)

• 产品编号：CST-5725SC      品牌：CST       原厂货号：5725SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的重组小鼠蛋白_His6IL-27 (m_His6IL-27)是异源二聚体，由EBI3 Tyr19-Pro228 (Accession# NP_056581)和p28 Phe29-Ser234 (Accession# NP_663611)通过 GGGSGGGSGGGSGGGS连接而成。此蛋白在人的293细胞中表达。重组的mIL-27蛋白在N-末端有His6标签，蛋白分子量据推算为50,551Da。DTT-还原的蛋白和未还原的蛋白作为55kDa的多肽而迁移。重组蛋白的N-末端序列通过测序来证实。6 μg 还原 (+) 和 非还原的(-) 重组m_His6IL-27通过SDS-PAGE检测，纯度大于97%，所有批次的纯度均高于97%。m_His6IL-27 的生物活性是通过病毒保护实验来确定的。每个批次的ED50在1-6 ng/ml之间。每微克m_His6IL-27内毒素含量小于0.01 ng。

有载体：每微克m_His6IL-27蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克m_His6IL-27蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-27是由p28和EBI3组成的异源二聚体(1)。抗原表达细胞包括单核细胞，巨噬细胞和树突状细胞，这些细胞都是IL-27最主要的来源(1)。IL-27是一个多向性的细胞因子，具有促炎症和抗炎症的双重活性。IL-27抑制TH17的应答并促进I型调节细胞分化的调控(2-4)。相反，IL-27在T细胞诱导的肠炎小鼠模型中显示了重要的促炎作用(5)。IL-27可能也具有抗病毒的活性(6)。IL-27发挥其作用主要是通过由WSX-1和gp130组成的异源二聚体受体(1,7)。IL-27诱导的信号通路包括Stat1和Stat3的磷酸化(1,7)。

原厂资料：

Source / Purification

Recombinant mouse His6IL-27 (mHis6IL-27) is a heterodimer that is composed of EBI3 Tyr19-Pro228 (Accession# NP_056581) linked to p28 Phe29-Ser234 (Accession# NP_663611) via the linker GGGSGGGSGGGSGGGS. mHis6IL-27 was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged mIL-27 has a calculated MW of 50,551. DTT-reduced and non-reduced protein migrate as 55 kDa polypeptides. The expected amino terminus of recombinant mHis6IL-27 was verified by amino acid sequencing.

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-27. All lots are greater than 97% pure.

Bioactivity

The bioactivity of mHis6IL-27 was determined in a virus protection assay. The ED50 of each lot is between 1-6 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg mHis6IL-27.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-27. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-27 is a heterodimer that consists of p28 and EBI3 (1). Antigen presenting cells including monocytes, macrophages, and dendritic cells are the primary sources of IL-27 (1). IL-27 is a pleiotropic cytokine with both pro- and anti-inflammatory activities. IL-27 suppresses TH17 responses and promotes type I regulatory cell differentiation (2-4). In contrast, IL-27 has been shown to play a pro-inflammatory role in a mouse model of T cell-induced colitis (5). IL-27 may also have anti-viral activities (6). IL-27 exerts its effects through a heterodimeric receptor consisting of WSX-1 and gp130 (1,7). IL-27-induced signaling results in Stat1 and Stat3 phosphorylation (1,7).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.