Human Cystatin C (hCystatin C)

• 产品编号：CST-5717LF      品牌：CST       原厂货号：5717LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

Cell Signaling Technology的重组人源Cystatin C(hCystatin C) 丝氨酸27-丙氨酸146（编号NP_000090）经人类293细胞表达。人源重组Cystatin C在N氨基末端不含甲基化，这种蛋白分子量为13347，DTT还原和未还原的蛋白游离物为分子量14kDa多肽。人源重组Cystatin C的预期N-氨基末端的SSPGK由氨基酸测序验证。每6 μg还原和未还原的重组hCystatin C由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。人源胱蛋白酶抑制剂C活性由评估其抑制木瓜蛋白酶调节的剪切荧光底物的能力测定。其每个批次的ED50在 1-12 ng/ml之间。每1 μg hCystatin C中内毒素含量低于0.01ng。带有载体：pH 7.2.每1ug hCystatin C溶于带有20ug BSA，pH7.2的PBS溶液经0.22 μm滤器过滤后冻干。不含载体：溶于PBS溶液经0.22 μm滤器过滤后冻干。Cystatin C 是西司他汀半胱氨酸蛋白酶抑制因子的西司他汀超家族的成员，分子量14kDa[1]。大部分细胞类型都分泌Cystatin C[1]。Cystatin C可抑制组织蛋白酶类，并因此通过抑制组织蛋白酶调节的肿瘤细胞侵袭而起着肿瘤抑制因子的作用[2]。此外，这种肿瘤抑制因子的功能也可归结于Cystatin C能阻断TGF-β1信号通路[2,3]。胱蛋白酶抑制剂C也能通过抑制组织蛋白酶类来调控抗原递呈[4]。胱蛋白酶抑制剂C也是肾功能异常的标志物[5]。

原厂资料：

Source / Purification

Recombinant human Cystatin C (hCystatin C) Ser27-Ala146 (Accession #NP_000090) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hCystatin C does not have a Met on the amino terminus and has a calculated MW of 13,347. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal SSPGK of recombinant hCystatin C was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCystatin C. All lots are greater than 98% pure.

Bioactivity

The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate. The ED50 of each lot 1-12 nM.

Endotoxin

Less than 0.01 ng endotoxin/1μg hCystatin C.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCystatin C. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Cystatin C is a 14 kDa member of the Cystatin superfamily of cysteine protease inhibitors (1). Most cell types secrete Cystatin C (1). Cystatin C inhibits cathepsins, and thereby may function as a tumor suppressor by inhibiting cathepsin mediated tumor cell invasion (2). In addition, this tumor suppressor function can also be attributed to Cystatin C''s ability to antagonize TGF-β1 signaling (2,3). Cystatin C may also modulate antigen presentation through its ability to inhibit cathepsins (4). Cystatin C is also a biomarker for kidney dysfunction (5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.