ApoM (8F12) Mouse mAb

• 产品编号：CST-5709S      品牌：CST       原厂货号：5709S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ApoM (8F12)小鼠单克隆抗体识别内源性的全部非糖基化和糖化ApoM蛋白。该单克隆抗体用纯化的人类ApoM重组片段免疫动物而制成。载脂蛋白是血浆脂蛋白，可在血液循环系统中转运血脂和胆固醇。乳糜微粒是一种含有极低密度脂蛋白（VLDL），中间密度脂蛋白（IDL），低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的载脂蛋白（1,2）。载脂蛋白M（ApoM）是一种新型的膜结合载脂蛋白，参与脂质运输。ApoM主要与HDL相互作用，调节其代谢（3）。它在肝细胞和肾近段小管细胞高表达。最近发现ApoM通过在上皮细胞运输S1P到S1P受体而作为HDL的血管保护成分发挥作用（4）。ApoM的单核苷酸多态性被发现与2型糖尿病有关（5）。

1. Kwiterovich, P.O. (2000) Am J Cardiol 86, 5L-10L.
2. Hussain, M.M. (2000) Atherosclerosis 148, 1-15.
3. Xu, N. and Dahlbäck, B. (1999) J Biol Chem 274, 31286-90.
4. Christoffersen, C. et al. (2011) Proc Natl Acad Sci U S A 108, 9613-8.
5. Zhou, J.W. et al. (2011) PLoS One 6, e17324.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

ApoM (8F12) Mouse mAb recognizes endogenous levels of both unglycosylated and glycosylated ApoM protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human ApoM protein.

Background

Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).Apolipoprotein M (ApoM) is a novel membrane bound apolipoprotein involved in lipid transport. ApoM primarily interacts with HDL and regulates its metabolism (3). It is highly expressed in hepatocytes and kidney proximal tubule cells. Recently, ApoM was shown to act as a vasculoprotective constituent of HDL by delivering S1P to the S1P receptor on endothelial cells (4). SNPs of ApoM have been associated with type 2 diabetes (5).

1. Kwiterovich, P.O. (2000) Am J Cardiol 86, 5L-10L.
2. Hussain, M.M. (2000) Atherosclerosis 148, 1-15.
3. Xu, N. and Dahlbäck, B. (1999) J Biol Chem 274, 31286-90.
4. Christoffersen, C. et al. (2011) Proc Natl Acad Sci U S A 108, 9613-8.
5. Zhou, J.W. et al. (2011) PLoS One 6, e17324.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.