PLCγ1 (D9H10) XP? Rabbit mAb

• 产品编号：CST-5690S      品牌：CST       原厂货号：5690S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PLCγ1(D9H10)XP兔单克隆抗体识别内源性的全部PLCγ1蛋白。该单克隆抗体用与人类PLCγ1蛋白中Leu1220位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。磷酸肌醇特异性磷脂酶C（PLC）在跨膜信号转导中扮演明显的作用。在对激素、生长因子和神经递质反应的过程中，PLC水解4,5-二磷酸磷酸酰肌醇，产生两个第二信使：1,4,5-三磷酸肌醇（IP3）和甘油二酯（DAG）（1）。至少四个PLC家族已被鉴定，分别为PLC-α、PLC-β、PLC-γ和PLC-δ。磷酸化是调节PLC活性的重要机制之一。PLCγ被受体和非受体酪氨酸激酶激活（2）。PLCγ与EGF和PDGF受体形成复合物，导致PLCγ的Tyr771、783和1245位点磷酸化（3）。Syk对Tyr783的磷酸化激活PLCγ1的酶活性（4）。PLCγ2参与B细胞抗原依赖的信号转导和血小板胶原依赖的信号转导。Btk或Lck对Tyr753、759、1197和1217的磷酸化与PLCγ2的活性相关（5,6）。

1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
2. Margolis, B. et al. (1989) Cell 57, 1101-7.
3. Kim, H.K. et al. (1991) Cell 65, 435-41.
4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

原厂资料：

Specificity / Sensitivity

PLCγ1 (D9H10) XP® Rabbit mAb recognizes endogenous levels of total PLCγ1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1220 of human PLCγ1 protein.

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
2. Margolis, B. et al. (1989) Cell 57, 1101-7.
3. Kim, H.K. et al. (1991) Cell 65, 435-41.
4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.