PPP2R5D (H5D12) Mouse mAb

• 产品编号：CST-5687S      品牌：CST       原厂货号：5687S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PPP2R5D (H5D12) Rabbit mAb 能够识别内源性PPP2R5D/PP2A, B'-δ/B56-δ 总蛋白。该抗体与PPP2R4和PPP2R2A蛋白发生交叉反应。该单克隆抗体是由重组的人源PPP2R5D蛋白氨基末端的肽段免疫动物生产的。蛋白磷酸酶2A亚基异构体R5-δ (PPP2R5D)属于丝氨酸/苏氨酸磷酸酶2A的调节亚基B的R5/B'/B56家族(1)。PPP2R5D (B-δ)和亚基B(B-α)的其它成分结构上相关。PPP2R5D缺少对于蛋白-蛋白相互作用很重要的WD-40重复序列(1-2)。结构差异可能有助于区分下游的效应(3)并指引PP2A的核定位(4)。现有的证据表明和B-α相比，PPP2R5D通过限制1型TGF-β受体、ALK4和ALK5的下游活性来调节TGF-β/Actividin/Nodal信号通路(3)。

1. McCright, B. and Virshup, D.M. (1995) J Biol Chem 270, 26123-8.
2. Neer, E.J. et al. (1994) Nature 371, 297-300.
3. Batut, J. et al. (2008) Development 135, 2927-37.
4. McCright, B. et al. (1996) J Biol Chem 271, 22081-9.

原厂资料：

Specificity / Sensitivity

PPP2R5D (H5D12) Rabbit mAb recognizes endogenous levels of total PPP2R5D/PP2A, B'-δ/B56-δ protein. This antibody cross-reacts with PPP2R4 and PPP2R2A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PPP2R5D protein.

Background

Protein Phosphatase 2A subunit isoform R5-δ (PPP2R5D) belongs to the R5/B'/B56 family of the regulatory subunit B of serine/threonine phosphatase 2A (1). PPP2R5D (B-δ) is structurally related to the other components of subunit B, B-α. PPP2R5D lacks the WD-40 repeats that are important for protein-protein interaction (1-2). Structural differences may contribute to distinct down stream effects (3) and directing PP2A nuclear localization (4). Current evidence suggests that in contrast to B-α, PPP2R5D modulates the TGF-β/Actividin/Nodal signaling pathways by restricting the downstream activities of the type I TGF-β receptors, ALK4, and ALK5 (3)

1. McCright, B. and Virshup, D.M. (1995) J Biol Chem 270, 26123-8.
2. Neer, E.J. et al. (1994) Nature 371, 297-300.
3. Batut, J. et al. (2008) Development 135, 2927-37.
4. McCright, B. et al. (1996) J Biol Chem 271, 22081-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.