HOP (D6E3) Rabbit mAb

• 产品编号：CST-5669S      品牌：CST       原厂货号：5669S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HOP (D6E3) Rabbit mAb兔单抗识别内源性的HOP总蛋白。此抗体不与其它热休克蛋白有交叉反应。该单克隆抗体用与人类HOP蛋白中Lys513位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。HOP也被称为应激诱导的磷酸化蛋白1（STIP），是大多数热休克蛋白、Hsp70和Hsp90的共分子伴侣，在早期受体复合物中出现（1,2）。通过与Hsp70和Hsp90的相互结合，Hop以接头蛋白的角色整合Hsp70和Hsp90的相互作用（3,4）。HOP是大量存在并且高度保守的蛋白质，包含三个tetratricopeptide重复（TPR）区段（TPR1、TPR2a和TPR2b）和两个DP重复区段（DP1和DP2），它们的功能目前还没有被研究清楚（5）。

1. Smith, D.F. et al. (1993) Mol Cell Biol 13, 869-76.
2. Smith, D.F. (1993) Mol Endocrinol 7, 1418-29.
3. Chen, S. and Smith, D.F. (1998) J Biol Chem 273, 35194-200.
4. Nelson, G.M. et al. (2003) Cell Stress Chaperones 8, 125-33.
5. Carrigan, P.E. et al. (2005) J Biol Chem 280, 8906-11.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine

Specificity / Sensitivity

HOP (D6E3) Rabbit mAb recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys513 of human HOP protein.

Background

HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).

1. Smith, D.F. et al. (1993) Mol Cell Biol 13, 869-76.
2. Smith, D.F. (1993) Mol Endocrinol 7, 1418-29.
3. Chen, S. and Smith, D.F. (1998) J Biol Chem 273, 35194-200.
4. Nelson, G.M. et al. (2003) Cell Stress Chaperones 8, 125-33.
5. Carrigan, P.E. et al. (2005) J Biol Chem 280, 8906-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.