Musashi-1 (D46A8) Rabbit mAb

• 产品编号：CST-5663S      品牌：CST       原厂货号：5663S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Musashi-1 (D46A8) XP® Rabbit mAb 兔单抗识别内源性的总Musashi-1蛋白。该单克隆抗体由合成的人类Musashi-1蛋白Met190位点附近肽段免疫动物而制成。Musashi-1和Musashi-2是RNA结合蛋白，通过调节靶mRNA的翻译在外胚层前体细胞的不对称细胞分裂中发挥作用。这两个成员都能增加Notch信号和抑制m-Numb的翻译；m-Numb是一种正向调节神经干细胞分化成神经元的蛋白质。因此，Musashi对神经干细胞的保存有贡献（1）。Musashi-1经常被用来作为神经前体细胞增殖的标记，它也在上皮干细胞，包括肠，乳腺干细胞中表达（2-4）。

1. Okano, H. et al. (2005) Exp. Cell Res. 306, 349-56.
2. Sakakibara, S. et al. (1996) Dev. Biol. 176, 230-42.
3. Potten, C.S. et al. (2003) Differentiation 71, 28-41.
4. Clarke, R.B. et al. (2005) Dev. Biol. 277, 443-56.

原厂资料：

Specificity / Sensitivity

Musashi-1 (D46A8) XP® Rabbit mAb detects endogenous levels of total Musashi-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met190 of human Musashi-1 protein.

Background

Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4).

1. Okano, H. et al. (2005) Exp. Cell Res. 306, 349-56.
2. Sakakibara, S. et al. (1996) Dev. Biol. 176, 230-42.
3. Potten, C.S. et al. (2003) Differentiation 71, 28-41.
4. Clarke, R.B. et al. (2005) Dev. Biol. 277, 443-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.