Non-phospho-STEP (Ser221) (D74H3) XP? Rabbit mAb

• 产品编号：CST-5659P      品牌：Cell Signaling Technology       原厂货号：5659P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Nonphospho-STEP (Ser221) (D74H3) XP® Rabbit mAb 兔单抗识别内源性的Ser221磷酸化的STEP61蛋白和Ser49磷酸化的STEP46蛋白。该单克隆抗体由合成的人类STEP61蛋白Ser221位点附近非磷酸化肽段免疫动物而制成。纹状体富集的磷酸酶（STEP，也称为PTPN5），是一种蛋白酪氨酸磷酸酶，在中枢神经系统多巴胺支配神经元中表达（1）。不同的剪接产生STEP的不同亚型：细胞质STEP46和膜相关STEP61。多巴胺激活D1受体和PKA，这反过来又磷酸化两种STEP异构体。STEP61的磷酸化发生在Ser160和Ser221，STEP46的则在Ser49（与STEP61 Ser221等同）（2）。NMDA受体介导的STEP激活是神经元ERK活性调节的重要机制（3）。此外，STEP参与调节NMDA受体和AMPAR转运（4,5）。由于其对认知功能的重要性，STEP可能在阿尔茨海默病中发挥作用（1）。STEP61的活性在Ser221（STEP46则为Ser49）磷酸化后由于与底物的亲和力下降而下调（2）。

1. Braithwaite, S.P. et al. (2006) Trends Neurosci 29, 452-8.
2. Paul, S. et al. (2000) J Neurosci 20, 5630-8.
3. Paul, S. et al. (2003) Nat Neurosci 6, 34-42.
4. Braithwaite, S.P. et al. (2006) Eur J Neurosci 23, 2847-56.
5. Zhang, Y. et al. (2008) J Neurosci 28, 10561-6.

原厂资料：

Specificity / Sensitivity

Nonphospho-STEP (Ser221) (D74H3) XP® Rabbit mAb detects endogenous levels of STEP61 protein only when dephosphorylated at Ser221 and of STEP46 protein when dephosphorylated at Ser49.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic nonphosphopeptide corresponding to residues surrounding Ser221 of human STEP61 protein.

Background

Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of ERK activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer disease (1).Activity of STEP61 is reduced upon phosphorylation of Ser221 (Ser49 of STEP46) due to lower affinity for its substrates (2).

1. Braithwaite, S.P. et al. (2006) Trends Neurosci 29, 452-8.
2. Paul, S. et al. (2000) J Neurosci 20, 5630-8.
3. Paul, S. et al. (2003) Nat Neurosci 6, 34-42.
4. Braithwaite, S.P. et al. (2006) Eur J Neurosci 23, 2847-56.
5. Zhang, Y. et al. (2008) J Neurosci 28, 10561-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody