PKD3/PKCν (D57E6) Rabbit mAb

• 产品编号：CST-5655S      品牌：CST       原厂货号：5655S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PKD3/PKCv(D57E6)兔单克隆抗体识别内源性的全部PKD3/PKCv蛋白。该单克隆抗体用与人类PKD3/PKCv蛋白中Ala880位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。PKCv，也被称为PKD3，是一种丝氨酸/苏氨酸蛋白激酶(PKC)家族激酶，在调节细胞分化和增殖中发挥关键作用。PKCv由890个氨基酸残基组成，与人类PKCμ有77.3％的相似性，与鼠PKD蛋白（PKCμ对应的鼠同源蛋白）有77.4%的相似性（1）。PKCv的mRNA在各种组织中广泛表达。PKCv有两个公认的甘油二酯结合的C1域，这表明它可能参与了一种新的甘油二酯介导的信号通路（2）。PKCv转位到细胞膜，被甘油二酯模拟的佛 12-豆蔻 13-醋酸激活。PKCv是B细胞受体（BCR）的一种重要的生理目标，并在BCR参与后表现出强大的激活属性（2）。G蛋白偶联受体激动剂通过蛋白激酶C（PKC）依赖的通路诱导PKCv快速激活，导致PKCv活性环的磷酸化。PKCv在细胞核和胞质中都存在，其这种连续性分布是其在细胞间隔间连续穿梭的结果，这种穿梭的机制需要入核受体和有力的CRM1出核通路（3）。用G蛋白偶联受体激动剂神经降压素刺激细胞，可以诱导一种快速的可逆的PKC依赖的PKCv的细胞膜转位。

1. Hayashi, A. et al. (1999) Biochim. Biophys. Acta. 1450, 99-106.
2. Matthews, S.A. et al. (2003) J. Biol. Chem. 278, 9086-91.
3. Rey, O. et al. (2003) J. Biol. Chem. 278, 23773-85.

原厂资料：

Specificity / Sensitivity

PKD3/PKCν (D57E6) Rabbit mAb recognizes endogenous levels of total PKD3/PKCν protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala880 of human PKD3/PKCν protein.

Background

PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation. PKCν is composed of 890 amino acid residues and has 77.3% similarity to human PKCμ (PKCμ) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCμ) (1). The PKCν mRNA is ubiquitously expressed in various tissues. PKCν has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCν is translocated to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induce a rapid activation of PKCν by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCν. PKCν is present both in the nucleus and cytoplasm and this distribution of PKCν results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKCν that is PKC-dependent.

1. Hayashi, A. et al. (1999) Biochim. Biophys. Acta. 1450, 99-106.
2. Matthews, S.A. et al. (2003) J. Biol. Chem. 278, 9086-91.
3. Rey, O. et al. (2003) J. Biol. Chem. 278, 23773-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.