Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb

• 产品编号：CST-5651S      品牌：CST       原厂货号：5651S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化-β-Catenin (丝氨酸552) (D8E11)兔单克抗只识别Ser552磷酸化的内源β-Catenin蛋白。此单克隆抗体是通过合成的肽段免疫动物产生。这种合成的肽段与人源β-catenin蛋白邻近Ser552氨基酸残基序列一致.β-catenin是Wnt信号通路下游的重要效应分子（1）。在脊椎动物体内它涉及到两个重要的生物过程：早期胚胎发育（2）和肿瘤发生（3）。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化（4-6）。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41，并促使其进一步被降解（7）。这些位点发生突变会提高β-catenin蛋白稳定性，并且已在多种癌细胞株中发现这些突变（8）。Akt 和 PKA均显示能磷酸化β-catenin的 552位丝氨酸。552位丝氨酸的磷酸化诱导β-catenin的核内累积并增加其转导活性[9-11].

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.
9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-9976.
10. Fang, D. et al. (2007) J Biol Chem 282, 11221-11229.
11. He, X.C. et al. (2007) Nat Genet 39, 189-198.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Xenopus, Zebrafish

Specificity / Sensitivity

Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb detects endogenous levels of β-catenin protein only when phosphorylated at Ser552.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin protein.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).Both Akt and PKA were shown to phosphorylate β-catenin at Ser552. Phosphorylation at Ser552 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-11).

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.
9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-9976.
10. Fang, D. et al. (2007) J Biol Chem 282, 11221-11229.
11. He, X.C. et al. (2007) Nat Genet 39, 189-198.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody