MTA1 (D17G10) Rabbit mAb

• 产品编号：CST-5646S      品牌：CST       原厂货号：5646S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MTA1 (D17G10) Rabbit mAb兔单抗能够检测内源性MTA1的总蛋白水平。通过合成的与人源MTA1蛋白Val632位点周围相应多肽片段去免疫动物从而制备出此单克隆抗体。MTA1 (metastasis associated gene 1)在转移性和非转移性腺癌细胞系的 cDNA文库的差异筛选中被鉴定(1)，并且随后被发现是核小体重塑和去乙酰化(NuRD)复合物的完整成员(2,3)。MTA1蛋白表达在缺氧条件下被上调，并且发现通过HIF-1α的稳定性能提高血管生成 (4,5)。MTA1在大范围的人源癌症中过表达，以及它的表达是与恶性肿瘤和肿瘤进展有关(6)。MTA1是一个本质的c-Myc转化的下游效应分子(7)。最近，MTA1被证明在DNA损伤反应中起着一定作用(8,9)。

1. Toh, Y. et al. (1994) J Biol Chem 269, 22958-63.
2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
3. Zhang, Y. et al. (1998) Cell 95, 279-89.
4. Yoo, Y.G. et al. (2006) EMBO J 25, 1231-41.
5. Moon, H.E. et al. (2006) Oncol Rep 16, 929-35.
6. Toh, Y. and Nicolson, G.L. (2009) Clin Exp Metastasis 26, 215-27.
7. Zhang, X.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13968-73.
8. Li, D.Q. et al. (2009) J Biol Chem 284, 34545-52.
9. Li, D.Q. et al. (2010) J Biol Chem 285, 10044-52.

原厂资料：

Specificity / Sensitivity

MTA1 (D17G10) Rabbit mAb detects endogenous levels of total MTA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val632 of human MTA1 protein.

Background

MTA1 (metastasis associated gene 1) was identified in a differential screening of a cDNA library of metastatic and nonmetastatic adenocarcinoma cell lines (1), and was subsequently found to be an integral member of the nucleosome remodeling and deacetylation (NuRD) complex (2,3). MTA1 expression is upregulated under hypoxic conditions and found to enhance angiogenesis through stabilization of HIF-1α (4,5). MTA1 is overexpressed in a wide range of human cancers, and its expression is associated with malignancy and tumor progression (6). MTA1 is an essential downstream effector of c-Myc transformation (7). Recently, MTA1 was demonstrated to play a role in DNA damage response (8,9).

1. Toh, Y. et al. (1994) J Biol Chem 269, 22958-63.
2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
3. Zhang, Y. et al. (1998) Cell 95, 279-89.
4. Yoo, Y.G. et al. (2006) EMBO J 25, 1231-41.
5. Moon, H.E. et al. (2006) Oncol Rep 16, 929-35.
6. Toh, Y. and Nicolson, G.L. (2009) Clin Exp Metastasis 26, 215-27.
7. Zhang, X.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13968-73.
8. Li, D.Q. et al. (2009) J Biol Chem 284, 34545-52.
9. Li, D.Q. et al. (2010) J Biol Chem 285, 10044-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.