Presenilin 1 (D39D1) Rabbit mAb

• 产品编号：CST-5643P      品牌：Cell Signaling Technology       原厂货号：5643P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Presenilin 1 (D39D1) Rabbit mAb 兔单抗识别内源性的Presenilin 1 总蛋白。该抗体也可以识别非特异的60kDa条带。该单克隆抗体由重组的人类Presenilin 1蛋白羧基末端蛋白免疫动物而制成。早老素1、2蛋白属于早老素家族的跨膜蛋白。早老素基因的突变已与阿尔茨海默病早期发病相关联，可能是由于早老相关的处理β-淀粉样蛋白的γ-分泌酶活性改变（1,2）。内源性的早老素主要以异二聚体复合物的形式存在，它来自同一基因裂解的氨基末端(34 kDa)和羧基末端(约20, 22, 23 kDa)片段（CTF）（2,3）。

1. Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
2. Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
3. Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.

原厂资料：

Specificity / Sensitivity

Presenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Presenilin 1 protein.

Background

Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin's associated γ-secretase activity for amyloid-β protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3).

1. Haass, C. and DeStrooper, B. (1999) Science 286, 916-919.
2. Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178.
3. Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody