A20/TNFAIP3 (D13H3) Rabbit mAb

• 产品编号：CST-5630S      品牌：CST       原厂货号：5630S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

A20/TNFAIP3 (D13H3) Rabbit mAb兔单抗能够检测内源水平A20/TNFAIP3蛋白总体水平。此单克隆抗体是通过合成对应人源A20/TNFAIP3 N-末端的融合蛋白来免疫动物而获得。此抗体是与Dr. Marc Schmidt-Supprian, Max Planck Institute of Biochemistry合作完成制备。A20, 也叫做TNF-α诱导蛋白3 (TNFAIP3), 是细胞活素诱导蛋白作用于抑制凋亡并激活NF-κB (1,2)。此蛋白最初是从人脐静脉内皮细胞分离的TNF-α诱导初级应答基因，它编码一个具有790氨基酸的蛋白其中包含7个Cys2/Cys2-锌指域(3)。在淋巴组织中，观察到A20属于组成型表达(4),但是，在受到TNF-α(3,5)、IL-1(3,5)、佛波酯(6)、LPS (7)诱导而产生炎症反应时，在很多细胞类型中都是短暂的表达(3,5)。A20的表达能够抵抗凋亡和NF-κB受到这些信号的激活,很可能是通过与TRAF (TNF 受体相关因子)家族成员(8,9)和NF-κB抑制蛋白ABIN相互作用 (10)。研究发现A20包含位点特异性泛素修饰活性对其生物功能有贡献(11,12)。 A20的N-末端包含对Lys63分支的去泛素化酶(DUB)活性, 如在TRAF6和 RIP中发现的, 同时，C-末端包含对Lys48分支泛素连接酶(E3)活性，Lys63 分支和 Lys48分支会位于同一个底物，并导致了他们的降解(12)。

1. Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
2. Lee, E.G. et al. (2000) Science 289, 2350-4.
3. Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
4. Tewari, M. et al. (1995) J Immunol 154, 1699-706.
5. Jäättelä, M. et al. (1996) J Immunol 156, 1166-73.
6. Laherty, C.D. et al. (1993) J Biol Chem 268, 5032-9.
7. Hu, X. et al. (1998) Blood 92, 2759-65.
8. Song, H.Y. et al. (1996) Proc Natl Acad Sci USA 93, 6721-5.
9. Heyninck, K. and Beyaert, R. (1999) FEBS Lett 442, 147-50.
10. Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82.
11. Evans, P.C. et al. (2004) Biochem J 378, 727-34.
12. Lin, S.C. et al. (2008) J Mol Biol 376, 526-40.

原厂资料：

Specificity / Sensitivity

A20/TNFAIP3 (D13H3) Rabbit mAb recognizes endogenous levels of total A20/TNFAIP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus terminus of human A20/TNFAIP3 protein. The antibody was prepared in collaboration with Dr. Marc Schmidt-Supprian, Max Planck Institute of Biochemistry.

Background

A20, also referred to as TNF-α-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-κB (1,2). It was first identified as a TNF-α inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs (3). Constitutive expression of A20 is observed in lymphoid tissues (4), but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-α (3,5), IL-1 (3,5), phorbol esters (6), and LPS (7). Expression of A20 can confer resistance to apoptosis and NF-κB activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members (8,9), and interaction with the NF-κB inhibiting protein ABIN (10). Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions (11,12). The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation (12).

1. Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
2. Lee, E.G. et al. (2000) Science 289, 2350-4.
3. Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
4. Tewari, M. et al. (1995) J Immunol 154, 1699-706.
5. Jäättelä, M. et al. (1996) J Immunol 156, 1166-73.
6. Laherty, C.D. et al. (1993) J Biol Chem 268, 5032-9.
7. Hu, X. et al. (1998) Blood 92, 2759-65.
8. Song, H.Y. et al. (1996) Proc Natl Acad Sci USA 93, 6721-5.
9. Heyninck, K. and Beyaert, R. (1999) FEBS Lett 442, 147-50.
10. Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82.
11. Evans, P.C. et al. (2004) Biochem J 378, 727-34.
12. Lin, S.C. et al. (2008) J Mol Biol 376, 526-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.