Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb

• 产品编号：CST-5617S      品牌：CST       原厂货号：5617S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb检测仅在Tyr376位点磷酸化的内源性ARHGAP42蛋白。通过人工合成人源ARHGAP42蛋白Tyr376位点周围相应的磷酸化片段去免疫动物从而制备单克隆抗体。ARHGAP家族基因的突变通过Rho/Rac/Cdc42-like GTPases异常调节导致致癌(2)。很少了解关于ARHGAP42蛋白的生理功能，其是ARHGAP家族成员之一。ARHGAP42蛋白包含一个蛋白序列中间的RhoGAP功能域、蛋白序列羧基末端SH3结合域、BAR结构域和序列氨基端PH功能域，这些表明该蛋白可能作为GTPase 激活因子和细胞骨架调节因子。

1. Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 333-8.
2. Katoh, M. and Katoh, M. (2004) Int J Oncol 25, 1201-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein.

Background

ARHGAP family members activate Rho GTPase proteins (1). Mutations of ARHGAP family genes lead to carcinogenesis through the dysregulation of Rho/Rac/Cdc42-like GTPases (2). Little is known about the biological function of ARHGAP42, a member of the ARHGAP family. ARHGAP42 contains a RhoGAP domain in the middle of its sequence, an SH3 binding domain at the C-terminal end of the protein sequence, as well as a BAR domain and a PH domain in the N-terminal region of the sequence, which indicate that the protein may function as GTPase activator and cytoskeletal adaptor.

1. Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 333-8.
2. Katoh, M. and Katoh, M. (2004) Int J Oncol 25, 1201-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody