β-Catenin (L54E2) Mouse mAb (Alexa Fluor? 555 Conjugate)

• 产品编号：CST-5612S      品牌：CST       原厂货号：5612S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

β-Catenin (L54E2)Mouse mAb鼠单抗(Alexa Fluor^® 555 偶联)识别内源性的β-catenin总蛋白.此单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与人β-catenin蛋白邻近羧基末端氨基酸残基序列一致.此CST抗体与Alexa Fluor555^® 荧光染料偶联，并在人类细胞中做自身免疫荧光分析检测分析。此抗体和未偶联的β-Catenin (L54E2) 鼠单抗(免疫荧光优选选择) #2677一样显示同样的物种交叉反应。β-catenin是Wnt信号通路下游的重要效应分子（1）。在脊椎动物体内它涉及到两个重要的生物过程：早期胚胎发育（2）和肿瘤发生（3）。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化（4-6）。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41，并促使其进一步被降解（7）。这些位点发生突变会提高β-catenin蛋白稳定性，并且已在多种癌细胞株中发现这些突变（8）。

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Pig

Specificity / Sensitivity

β-Catenin (L54E2) Mouse mAb (Alexa Fluor^® 555 Conjugate) detects endogenous levels of total β-catenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β-catenin protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 555 fluorescent dye and tested in-house for immunofluorescence in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze