MARCKS (D88D11) XP? Rabbit mAb

• 产品编号：CST-5607P      品牌：Cell Signaling Technology       原厂货号：5607P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MARCKS (D88D11) XP兔单克隆抗体识别内源性的全部MARCKS蛋白。该单克隆抗体用与人类MARCKS蛋白中Ala245位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。豆寇酰化富含丙氨酸C-激酶底物（MARCKS）是一种重要的PKC底物，在多种细胞类型中表达。 MARCKS与细胞活力，细胞粘附，吞噬，膜运输和有丝分裂有关（1）。PKC响应生长因子和氧化应激磷酸化MARCKS的丝氨酸（159，163，167和170位），调节钙/钙调蛋白结合和MARCKS丝状（F）-肌动蛋白交联反应活性（2-4）。PKC磷酸化作用导致MARCKS从细胞膜到细胞质迁移(5)。

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

原厂资料：

Specificity / Sensitivity

MARCKS (D88D11) XP® Rabbit mAb recognizes endogenous levels of total MARCKS protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala245 of human MARCKS protein.

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.