βIG-H3 (D31B8) XP? Rabbit mAb

• 产品编号：CST-5601P      品牌：Cell Signaling Technology       原厂货号：5601P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

βIG-H3 (D31B8) XP®兔单抗可以识别内源性总的βIG-H3蛋白。合成对应人βIG-H3蛋白羧基端序列的多肽免疫动物获得单克隆抗体。βIG-H3（TGFBI/RGD-CAP/Kerato-epithelin）是TGF-β诱导的一个包括683个氨基酸的分泌蛋白在细胞粘附，分化和凋亡过程中发挥功能（1-4）。βIG-H3包括一个内部富集半胱氨酸的EMI结构域跟随着4个成束蛋白1结构域和一个羧基端RGD结构域（1,2）。它可以与整合素以及一系列细胞外基质（ECM）蛋白包括胶原，纤连蛋白和层粘连蛋白结合发挥细胞粘附作用（5-7）。ECM βIG-H3在多种组织中表达（8-12）。βIG-H3基因突变及蛋白水平升高主要与角膜营养不良密切相关（13）。

1. Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22.
2. Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84.
3. Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14.
4. Kim, J.E. et al. (2003) Oncogene 22, 2045-53.
5. Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61.
6. Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9.
7. Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41.
8. Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96.
9. Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9.
10. Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62.
11. Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900.
12. LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9.
13. Munier, F.L. et al. (1997) Nat Genet 15, 247-51.

原厂资料：

Specificity / Sensitivity

βIG-H3 (D31B8) XP® Rabbit mAb detects endogenous levels of total βIG-H3

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human βIG-H3 protein.

Background

βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).

1. Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22.
2. Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84.
3. Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14.
4. Kim, J.E. et al. (2003) Oncogene 22, 2045-53.
5. Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61.
6. Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9.
7. Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41.
8. Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96.
9. Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9.
10. Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62.
11. Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900.
12. LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9.
13. Munier, F.L. et al. (1997) Nat Genet 15, 247-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.