Anti-biotin (D5A7) Rabbit mAb

• 产品编号：CST-5597S      品牌：CST       原厂货号：5597S
• 产品分类：抗体 > 一抗 > 其它类型一抗
• 应用分类：

描述：

抗生物素（D5A7）兔源单克隆抗体可以识别与蛋白、肽、寡核苷酸或固相基质上的生物素。单克隆抗体是用生物素化的蛋白免疫动物生产的。生物素是一种溶于水的维生素B复合体（维生素H或B7），在多种代谢功能中可以作为辅酶。它将二氧化碳转化为为5-羧化酶，参与TCA循环和糖的生成。此外,所有的细胞都需要生物素来维持细胞的增生，产生脂肪酸和代谢脂肪和氨基酸(1)。生物素的循环及对组蛋白的吸附是由生物素酶来催化的。组蛋白的生物素化参与调节端粒的消耗及细胞的衰老。生物素化的缺陷是一种很少见的遗传疾病，如果不及时治疗可以引起神经系统和皮肤的症状（2）。生物素与它的配体抗生素蛋白具有很高的亲和力。生物素结合位点的结构的复杂性和不规则性使得它只于生物素结合，表现出抗生素蛋白-生物素复合体的高特异性（3）。它的显著的亲和力已经被应用于很多免疫方法中比如ELISA、流式细胞术、免疫荧光、原位杂交、亲合色谱法和免疫组化。 抗生物素（D5A7）兔源单克隆抗体可以作为抗生素蛋白的替代品以减少背景和增加信号强度。

1. Zempleni, J. et al. (2010) Biofactors 35, 36-46.
2. Pindolia, K. et al. (2011) Mol Genet Metab 102, 161-9.
3. Reznik, G.O. et al. (1998) Proc Natl Acad Sci USA 95, 13525-13530.

原厂资料：

Specificity / Sensitivity

Anti-Biotin (D5A7) Rabbit mAb recognizes biotin attached to proteins, peptides, oligonucleotides, or solid matrices.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a biotinylated protein.

Background

Biotin is a water-soluble B complex vitamin (vitamin H or B7) that serves as a coenzyme in various metabolic functions. It transfers carbon dioxide for five carboxylase enzymes and is involved in the TCA cycle and gluconeogenesis. In addition, all cells require biotin for cell proliferation, production of fatty acids, and metabolism of fats and amino acids (1). Biotin recycling and attachment to histone proteins is catalyzed by an enzyme named biotinidase. Histone biotinylation is involved in regulating telomere attrition and cellular senescence. Biotinidase deficiency is a rare genetic disorder that can cause neurological and cutaneous symptoms if left untreated (2). Biotin has a high affinity for its natural ligand, avidin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the avidin-biotin complexes (3). This remarkable affinity has been exploited for numerous immunoassays including ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, Affinity Chromatography, and Immunohistochemistry. Anti-Biotin (D5A7) Rabbit mAb can be used as an alternative to avidins in order to minimize background and maximize signal intensity.

1. Zempleni, J. et al. (2010) Biofactors 35, 36-46.
2. Pindolia, K. et al. (2011) Mol Genet Metab 102, 161-9.
3. Reznik, G.O. et al. (1998) Proc Natl Acad Sci USA 95, 13525-13530.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA, 50% Glycerol and less than 0.02% sodium
azide. Store at -20C. Do not aliquot antibody