Human Neurotrophin-4 (hNT-4)

• 产品编号：CST-5592LC      品牌：CST       原厂货号：5592LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > Neurotrophin神经营养因子
• 应用分类：

描述：

重组人神经营养因子4（hNT-4）Gly81-Ala210（Accession #NP_006170）由CST公司利用大肠杆菌生产。重组hNT-4在氨基末端没有Met，分子量为 13,923。DTT还原和非还原的蛋白电泳可见14kDa的多肽。重组hNT-4的氨基末端GVSET使用氨基酸测序验证。6μg还原和非还原的重组hNT-4经SDS-PAGE验证纯度>98%。 所有批次纯度超过98%。hNT-4的活性通过在TrkB转染的NIH/3T3细胞中测定磷酸化的TrkB来评估。观察到的ED50为1-4ng/ml。内毒素小于0.01ng/1 μg hHNT-4。有载体：冻干自经0.22μm孔过滤的20 mM 柠檬酸盐，pH为3.0，每μg hNT-4含有100mM NaCl和20μg BSA。无载体：冻干自经0.22μm孔过滤的20 mM柠檬酸盐，pH为3.0，含100 mM NaCl。NT-4是一种与神经营养因子家族结构相关的蛋白质，其中包括β-神经生长因子，BDNF和NT-3（1）。NT-4在某些类型的细胞和组织中表达，包括神经细胞，正常乳腺上皮细胞，黑色素细胞，激活的T细胞和粒细胞（1-5）。NT-4是外周感觉神经元发育所需要的（6,7）。NT-4可能对长时程记忆的形成很重要（8）。黑色素细胞NT-4表达增加促进细胞增殖和转移（5）。NT-4被细胞以前体蛋白的形式分泌，被裂解成成熟的形式（1）。NT-4信号是通过两种不同的受体介导，它们是神经营养因子受体p75NTR和Trk酪氨酸激酶受体TrkB。虽然所有的神经营养因子都能结合p75NTR受体，NT-4能优先结合TrkB受体（1）。

原厂资料：

Source / Purification

Recombinant human Neurotrophin-4 (hNT-4) Gly81-Ala210 (Accession #NP_006170) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hNT-4 does not have a Met on the amino terminus and has a calculated MW of 13,923. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal GVSET of recombinant hNT-4 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-4. All lots are greater than 98% pure.

Bioactivity

The activity of hNT-4 was assessed by quatification of phospho-TrkB in TrkB transfected NIH/3T3 cells. The observed ED50 values are between 1-4 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hNT-4.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hNT-4. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

NT-4 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-3 (1). NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5). NT-4 is required for the development of peripheral sensory neurons (6,7). NT-4 may be important for the development of long term memory (8). Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5). NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB. While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.