Phospho-Tuberin/TSC2 (Ser1387) Antibody

• 产品编号：CST-5584S      品牌：CST       原厂货号：5584S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tuberin/TSC2 (Ser1387) Antibody可以检测出在ser1387位点磷酸化的内源性总马铃薯球蛋白（Tuberin），此抗体可以与140kD的未知蛋白发生交叉反应。多克隆抗体是采用人类马铃薯球蛋白（Tuberin）Ser1387周围残基相对应的合成磷酸肽段免疫动物生产的，抗体采用蛋白A和肽亲和层析法纯化的。马铃薯球蛋白（Tuberin）是TSC2肿瘤抑制基因的产物，是细胞增殖和肿瘤发展的重要调节因子（1）。TSC2或与之相关的TSC1（hamartin）的突变可以引起结节硬化复合体TSC（tuberous sclerosis complex），是一种常染色体显性遗传病，其特点为发生多种广泛的非恶性肿瘤（2）。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化（3）。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性（3-5）。另外，tuberin可以抑制mTOR（mammalian target of rapamycin）， 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白（4E-BP1，一种转录起始抑制因子）的活性，并最终抑制翻译（3,6,7）。Tuberin被AMPK 在Ser1387位点磷酸化是使细胞在能量供应减少情况下对细胞大小控制以保护细胞不凋亡（8）。 另外，在Ser1387磷酸化为在上游位点(Ser1383, Ser1379 and Ser1375)被GSK3磷酸化做好准备，参与Wnt信号通路（9）。

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.
8. Inoki, K. et al. (2003) Cell 115, 577-590.
9. Inoki, K. et al. (2006) Cell 126, 955-968.

原厂资料：

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kD protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1387 of human tuberin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis (8). Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling (9).

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.
8. Inoki, K. et al. (2003) Cell 115, 577-590.
9. Inoki, K. et al. (2006) Cell 126, 955-968.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody