DLST Antibody

• 产品编号：CST-5556S      品牌：CST       原厂货号：5556S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DLST Antibody用于检测内源性DLST总蛋白水平。该多克隆抗体用与人类DLST中Val188位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。α酮戊二酸脱氢酶复体物是线粒体柠檬酸循环中的限速酶（1）。二氢硫辛酰胺－琥珀酰转移酶是复合物中的关键亚基（2）。该基因的突变会导致家族性的阿尔茨海默病（1）。DLST的数值减少会增加活性氧簇的产生，提示它可能在氧化应激中发挥作用（2）。DLST缺失小鼠线粒体氧化应激增加，并且会导致阿尔茨海默病的发生（2）。

1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

原厂资料：

Specificity / Sensitivity

DLST Antibody detects endogenous levels of total DLST protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val188 of human DLST protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle in mitochondria (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Mutations in the DLST gene may be responsible for the familial Alzheimer disease (1). Reduction of DLST increases the generation of reactive oxygen species production suggesting its role in oxidative stress (2). Deficiency of DLST in mice was suggested to cause increased oxidative stress in mitochondria and is involved in the pathogenesis of Alzheimer disease (2).

1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.