SMC4 (D14E2) Rabbit mAb

• 产品编号：CST-5547S      品牌：CST       原厂货号：5547S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源性SMC2总蛋白的水平。该抗体不与其它的SMC蛋白如SMC1，SMC3和SMC4交叉反应。该单克隆抗体是由合成的针对人SMC4蛋白的95位酪氨酸的肽段免疫动物生产的。染色体结构维持蛋白SMC2和SMC4是凝集蛋白复合体的亚基，该复合体有助于染色体聚集和维持分裂后期染色体向两极分化时的致密化(1-3)。除了调节染色体聚集外，凝集蛋白也是一个染色体结构调节子并且具有调节基因表达和DNA修复的功能。SMC蛋白含有一个隶属于ABC ATPase超家族的标志性二元ATPase结构域，由氨基末端Walker A模体核苷酸结合结构域和羧基末端的Walker B模体催化结构域组成，它们通过相互作用形成功能性的ATPase(1-3)。两个ATPase结构域通过两个卷曲螺旋结构域连接，一个核心的促使SMC蛋白相互作用的铰链区可以分离它们。在凝集蛋白复合体存在的情况下，SMC2和SMC4相互作用形成功能性的ATPase参与染色质聚集。然而，染色体结构调节的具体机制还在研究中。除了SMC蛋白以外，凝集蛋白还包含3个辅助亚基能够调节凝集蛋白的ATPase活性。高等真核细胞含有两个特异性的凝集蛋白复合体(凝集蛋白 I and II)，都包含SMC2和SMC4(1-3)。凝集蛋白I也包含三个辅助亚基CAP-D2, CAP-G 和 CAP-H，而condensin II包含相关的辅助蛋白CAP-D3, CAP-G2 and CAP-H2。这两个复合体在细胞周期和染色体上呈现出不同的定位模式，且都为成功的有丝分裂所需，表明了其独特的功能(1-3)。

1. Losada, A. and Hirano, T. (2005) Genes Dev 19, 1269-87.
2. Hudson, D.F. et al. (2009) Chromosome Res 17, 131-44.
3. Legagneux, V. et al. (2004) Biol Cell 96, 201-13.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Bovine

Specificity / Sensitivity

SMC4 (D14E2) Rabbit mAb detects endogenous levels of total SMC4 protein. The antibody does not cross-react with other SMC proteins, including SMC1, SMC2 and SMC3. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr95 of human SMC4 protein. 

Background

Structural maintenance of chromosomes 2 (SMC2) and 4 (SMC4) proteins are subunits of the condensin complex, which enables chromosome condensation and maintains the compaction of chromosomes as they separate to opposite poles during anaphase (1-3). In addition to regulating chromosome condensation, condensin is a general regulator of chromosome architecture and may function to regulate gene expression and DNA repair. SMC proteins contain a hallmark bipartite ATPase domain of the ABC ATPase superfamily, which consists of an N-terminal Walker A motif nucleotide-binding domain and C-terminal Walker B motif catalytic domain that interact to form a functional ATPase (1-3). The two ATPase domains are connected by two coiled coil domains separated by a central hinge region that facilitates protein-protein interactions between partnering SMC proteins. In the case of the condensin complex, SMC2 and SMC4 interact to form a functional ATPase required for chromatin condensation; however, the mechanism by which this ATPase activity regulates chromsome architecture is still being determined. In addition to SMC proteins, condensin contains three auxiliary subunits, which function to regulate condensin ATPase activity. Higher eukaryotes contain two distinct condensin complexes (condensin I and II), both of which contain SMC2 and SMC4 (1-3). Condensin I also contains the auxiliary subunits CAP-D2, CAP-G and CAP-H, while condensin II contains the related auxiliary proteins CAP-D3, CAP-G2 and CAP-H2. The two condensin complexes show different localization patterns during the cell cycle and on chromosomes and both are required for successful mitosis, suggesting distinct functions for each complex (1-3). 

1. Losada, A. and Hirano, T. (2005) Genes Dev 19, 1269-87.
2. Hudson, D.F. et al. (2009) Chromosome Res 17, 131-44.
3. Legagneux, V. et al. (2004) Biol Cell 96, 201-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.