hnRNP A0 (D8A3) XP? Rabbit mAb

• 产品编号：CST-5545P      品牌：Cell Signaling Technology       原厂货号：5545P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP A0 (D8A3) XP® Rabbit mAb兔单抗检测内源性hnRNP A0总蛋白水平。该抗体不与其它hnRNP家族成员发生交叉反应。通过人工合成人源hnRNP A0蛋白Leu114位点周围序列相应的多肽片段去免疫动物从而制备单克隆抗体。Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0)是RNA结合蛋白相关的hnRNP A/B家族成员。该蛋白能够结合pre-mRNA，同时涉及到RNA的加工、新陈代谢和细胞核pre-mRNA转录本的转运(1)。hnRNP蛋白的 A/B亚家族包含有A1、A2/B1、A3和A0。 LPS 刺激小鼠巨噬细胞后，hnRNP A0蛋白通过MAPKAPK-2通路在Ser84位点发生磷酸化，且这也可能在TNF-α 刺激翻译中扮演重要角色 (2)。

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rousseau, S. et al. (2002) EMBO J 21, 6505-14.

原厂资料：

Specificity / Sensitivity

hnRNP A0 (D8A3) XP® Rabbit mAb recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu114 of human hnRNP A0 protein.

Background

Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rousseau, S. et al. (2002) EMBO J 21, 6505-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.