TGF-beta Receptor III (D11G10) Rabbit mAb

• 产品编号：CST-5544S      品牌：CST       原厂货号：5544S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TGF-β 受体 III (D11G10)Rabbit mAb 兔单抗识别内源性的TGF-β 受体 III总蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源TGF-β 受体 III蛋白的胞外结构域氨基酸残基序列一致。转化生长因子β (TGF-β)超家族成员是一类关键的细胞增殖、分化、发育、形态形成和疾病病理机制的调控因子[1-4]。TGF-β通过三种细胞表面受体I型 (RI), II 型(RII) 和III型 (RIII)传递信号。I型 和 II 型受体是丝氨酸/苏氨酸激酶，形成异侧复合物。在配体结合的刺激下，II 型受体与I型受体形成稳定复合物使I型受体激酶磷酸化并激活[5]。III型受体也称β聚糖，是一种跨膜糖蛋白，它有一个大的胞外结构域与TGF-β有高的亲和结合性但缺乏一个胞质信号结构域[6,7]。III型受体表达能通过递呈配体到信号复合物来调控TGF-β信号。唯一所知的TGF-β直接信号效应器是Smad家族蛋白，这类蛋白从细胞表面直接转导信号至细胞核内来调控靶基因的转录[8,9]。

1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
7. Wang, X.F. et al. (1991) Cell 67, 797-805.
8. Derynck, R. et al. (1998) Cell 95, 737-740.
9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.

原厂资料：

Specificity / Sensitivity

TGF-β Receptor III (D11G10) Rabbit mAb detects endogenous levels of total TGF-β Receptor III protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of TGF-β receptor III protein.

Background

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).

1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
7. Wang, X.F. et al. (1991) Cell 67, 797-805.
8. Derynck, R. et al. (1998) Cell 95, 737-740.
9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody