S100A10 (4E7E10) Mouse mAb

• 产品编号：CST-5529S      品牌：CST       原厂货号：5529S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

S100A10 (4E7E10)小鼠单克隆抗体识别内源性的全部S100A10蛋白。此抗体尚未发现与S100家族的其它蛋白有交叉反应。该单克隆抗体用大肠杆菌上表达的人S100A10蛋白重组片段免疫动物而制成。该抗体使用蛋白G色谱纯化而得。尽管S100蛋白分子量相对较小（8-12 kDa），结构简单，但仍可调节多种细胞过程，如细胞的生长和迁移，细胞周期，转录和分化。至今为止，已确定包括S100A1-S100A18，毛透明蛋白，丝聚合蛋白, 中间丝相关蛋白, S100P和S100Z在内的25名成员，成为EF-手形蛋白和钙结合蛋白家族中最大的群体。有趣的是，14个S100基因都聚集在人类染色体1q21上，是一个基因组不稳定的区域。研究表明，异常的S100蛋白表达和癌症发展之间存在显著的相关性。在多种组织类型中，S100蛋白主要介导免疫反应，但也参与神经元的发育（1-4）。每个S100单体有两个EF-手形基序，最多可以结合2个钙分子（或某些情况下，其他二价阳离子）。结构证据表明，S100蛋白形成反平行的同源或异源二聚体，以钙依赖性（有时是与钙无关的）的方式协调结合配偶体。虽然结构和功能相似，个别成员的分布有组织特异性，局限在特定的细胞区室，表现出独特的蛋白结合伴侣，在不同的信号转导通路中各自扮演一个特定的角色。除了细胞内的作用，一些S100蛋白作为胞外配体的受体起作用或被分泌出来并具有细胞因子样的活性（1-4）。S100A10（也被称作P11或calpactin 1的轻链）与Annexin-A2（ANXA2）形成异四聚体，通过与细胞膜（通过ANXA2）和各种蛋白质如SNARE复合体或肌动蛋白的相互作用，作为细胞膜和肌动蛋白细胞骨架之间的桥梁（5-7）。有假设认为S100A10在神经信号转导中发挥关键作用，因其可以与神经递质受体和神经元特异性离子通道如5-HT1B，TRPV5，ASIC1，Task1和NaV1.8相互作用（8-10）。近来，S100A10也显示出可通过其结合并传递递质受体样信号对纤维蛋白溶酶原做出反应，调节巨嗜细胞的活性和入侵(11,12)。

1. Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68.
2. Donato, R. (2003) Microsc Res Tech 60, 540-51.
3. Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22.
4. Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14.
5. Illien, F. et al. (2010) Biochim Biophys Acta 1798, 1790-6.
6. Umbrecht-Jenck, E. et al. (2010) Traffic 11, 958-71.
7. Jung, M.J. et al. (2010) Exp Cell Res 316, 1234-40.
8. van de Graaf, S.F. et al. (2003) EMBO J 22, 1478-87.
9. Girard, C. et al. (2002) EMBO J 21, 4439-48.
10. Donier, E. et al. (2005) J Biol Chem 280, 38666-72.
11. O'Connell, P.A. et al. (2010) Blood 116, 1136-46.
12. Swisher, J.F. et al. (2010) Blood 115, 549-58.

原厂资料：

Specificity / Sensitivity

S100A10 (4E7E10) Mouse mAb recognizes endogenous levels of total S100A10 protein. This antibody is not known to react with other S100 family proteins.

Source / Purification

Monoclonal antibody is generated by immunizing animals with a recombinant fragment of human S100A10 protein expressed in E. coli. The antibody is affinity purified by Protein G chromatography.

Background

Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). 
 
 Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners that suggest each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4).S100A10 (alternately known as p11 or calpactin 1 light chain) forms a constitutive heterotetramer with annexin-A2 (ANXA2) and may act as a bridge between the plasma membrane and actin cytoskeleton via interactions with the plasma membrane (via ANXA2) and various protein partners such as the SNARE complex or actin (5-7). S100A10 has been hypothesized to play a critical role in neuronal signaling due to its interaction and regulation of neurotransmitter receptors and neuron-specific ion channels such as 5-HT1B, TRPV5, ASIC1, TASK1 and NaV1.8 (8-10). More recently, S100A10 has also been shown to modulate macrophage activation and invasion via its ability to bind and transmit receptor-like signals in response to plasminogen (11,12).

1. Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68.
2. Donato, R. (2003) Microsc Res Tech 60, 540-51.
3. Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22.
4. Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14.
5. Illien, F. et al. (2010) Biochim Biophys Acta 1798, 1790-6.
6. Umbrecht-Jenck, E. et al. (2010) Traffic 11, 958-71.
7. Jung, M.J. et al. (2010) Exp Cell Res 316, 1234-40.
8. van de Graaf, S.F. et al. (2003) EMBO J 22, 1478-87.
9. Girard, C. et al. (2002) EMBO J 21, 4439-48.
10. Donier, E. et al. (2005) J Biol Chem 280, 38666-72.
11. O'Connell, P.A. et al. (2010) Blood 116, 1136-46.
12. Swisher, J.F. et al. (2010) Blood 115, 549-58.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.