Plexin A3 (D2G12) Rabbit mAb

• 产品编号：CST-5512S      品牌：CST       原厂货号：5512S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Plexin A3 (D2G12)Rabbit mAb 兔单抗识别内源性的Plexin A3总蛋白。Plexin A3 (D2G12)Rabbit mAb 兔单抗还可识别其它家族的蛋白质。该单克隆抗体由合成的人类Plexin A3蛋白Val440位点附近肽段免疫动物而制成。Class 3 secreted semaphorin (Sema3A)是多种轴突上的神经生长导向因子。因此，它可以诱导微丝发生戏剧性的再分布和解聚，导致生长锥崩塌。Plexins是单次跨膜信号转导蛋白，包括Plexin A1，A2，A3和A4。Plexins可以与neuropilin-1、neuropilin-2和细胞黏附蛋白L1形成功能性的semaphorin受体（1,2）。GTP酶Rnd1结合Plexin A1的细胞质结构域，触发细胞骨架崩溃。相反，GTP酶RndD阻止Rnd1-介导的Plexin A1的活性和Sema3A所致交感神经轴突的排斥（3）。Plexin A3和A4都介导感觉和交感神经元的第三类semaphorins反应。 特别是Plexin A4通过neuropilin-1对Sema 3A起反应，Plexin A3通过neuropilin-2对Sema 3F起反应（4）。

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. Yaron, A. et al. (2005) Neuron 45, 513-23.

原厂资料：

Specificity / Sensitivity

Plexin A3 (D2G12) Rabbit mAb detects endogenous levels of total Plexin A3 protein. Plexin A3 (D2G12) Rabbit mAb may cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Val440 of human Plexin A3.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (4).

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. Yaron, A. et al. (2005) Neuron 45, 513-23.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody