Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb

• 产品编号：CST-5500S      品牌：CST       原厂货号：5500S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-RCC1 (Ser11) (D18B5)Rabbit mAb可以识别11位丝氨酸被磷酸化后的RCC1蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人RCC1蛋白11位丝氨酸附近氨基酸一致。核膜形成，有丝分裂纺锤体的聚集以及核转运过程都涉及了Ras家族小GTPase Ran蛋白（1.2）。和其他的小GTPases类似，Ran结合GTP时出于激活状态，结合GDP时出于失活状态。细胞核RanGTP浓度由鸟嘌呤核苷酸交换因子（GEF）决定，GEF能够催化结合GTP以取代GDP。chromatin condensation1 （RCC1）的调控因子是唯一已知的RanGEF（3）。RCC1在细胞周期中动态地与染色质结合，这种结合对于有丝分裂的正常进行是必须的（4，5）。RCC1与染色质的适当协作由氨基末端磷酸化（5,6）和甲基化（7）调控。凋亡过程中组蛋白修饰能够改变RanGTP的水平以调节RCC1最终调控细胞核的输入（8）。在有死费列过程中哦该，RCC1可能是被细胞周期蛋白B/cdc2磷酸化第11位丝氨酸的（9-11）。该磷酸化影响RCC1与染色质的结合以及RCC1 RanGEF的功能（6）。

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Moore, W. et al. (2002) Curr Biol 12, 1442-7.
4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.

原厂资料：

Specificity / Sensitivity

Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb recognizes endogenous levels of RCC1 protein only when phosphorylated at Ser11.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser11 of human RCC1 protein.

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 (RCC1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity (6).

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Moore, W. et al. (2002) Curr Biol 12, 1442-7.
4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.