Human Transforming Growth Factor α (hTGF-α)

• 产品编号：CST-5495SC      品牌：CST       原厂货号：5495SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

CST公司生产的人重组蛋白TGF-α (hTGF-α) Val40-Ala89 (Accession #NP_003227)是从大肠杆菌细胞表达而来。基于氨基酸测序结果，50%以上的重组hTGF-α蛋白在N-末端有Met标签Val40 (MVVSH)并且分子量为5,683Da。其余的从Val40 (VVSHF)开始表达。6 μg 还原 (+) 和 非还原的(-) 重组hTGF-α通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hTGF-α的生物活性是通过MCF-10A 细胞增殖能力的实验来确定的。每个批次的ED50在0.25-1.0 ng/ml之间。

有载体: 每微克hTGF-α蛋白溶解在包含100 mM NaCl和 20 μg BSA的20 mM 柠檬酸盐(pH 3.0)溶液中，通过 0.22 μm 滤膜并冻干。

无载体：每微克hTGF-α蛋白溶解在包含100 mM NaCl20 mM 柠檬酸盐(pH 3.0)溶液中，通过 0.22 μm 滤膜并冻干。

TGF-α是EGF蛋白家族的成员之一，主要通过上皮细胞、角化细胞、巨噬细胞和很多肿瘤起源的细胞系表达产生(1-4)。可溶性的TGF-α主要是通过水解酶对跨膜的前体剪切而产生的(5)。TGF-α促进细胞增殖和分化成包括癌细胞和神经细胞的一系列的细胞类型(1,4,6)。TGF-α结合到EGFR/ErbB1 并诱导受体的二聚化和酪氨酸激酶的激活(7)。TGF-α激活MAPK通路并有Stat3和Stat5的参与 (1,6)。增减TGF-α表达的水平与包括乳腺癌的很多癌症有关(1,8,9)。

原厂资料：

Source / Purification

Recombinant human TGF-α (hTGF-α) Val40-Ala89 (Accession #NP_003227) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 50% of recombinant hTGF-α has a Met on the amino-terminal Val40 (MVVSH) and has a calculated MW of 5,683. The remainder starts at Val40 (VVSHF). DTT-reduced and non-reduced protein migrate as 5 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-α. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hTGF-α was determined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 0.25-1.0 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hTGF-α.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hTGF-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

TGF-α is a member of the EGF family of proteins and is expressed by epithelial cells, keratinocytes, macrophages, and many tumor-derived cell lines (1-4). Soluble TGF-α is produced by proteolytic cleavage of a transmembrane precursor (5). TGF-α promotes cell proliferation and differentiation of a diverse array of cell types including cancer and neural cells (1,4,6). TGF-α binds to EGFR/ErbB1 and induces receptor dimerization and tyrosine kinase activation (7). TGF-α activates the MAPK pathways and involves Stat3 and Stat5 (1,6). Increased levels of TGF-α expression are associated with many cancers including breast cancer (1,8,9).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.